Primary cancer cell expansion medium suggestions?

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Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

The algal cell always gather together when the cells concentration is high and those cells would block the microfluidic chips. How can i handle it?

I want to pump a high concentration algal cells into microfluidic chip, however those cells are always gather together forming a clusters to block the connection between Teflon tube and...

26 October 2020 5,366 2 View

What kind of small mobile robots are available on market to undertake multi-robot research?

Dear All, What kind of small mobile robots are available in market to undertake multi-robot research? Would you have anything to be recommended? Thanks! Chaomin Luo ~~~ Chaomin Luo,...

04 October 2020 5,173 5 View

Why some structural equation models are all observation variables?

Hello everyone. Recently, when I searched the literature about SEM, I found some problems that I couldn't solve. I found that the models established in some studies are all observation variables...

16 July 2020 3,279 3 View

2D protein gel system?

Want to set up a 2D protein gel system. Can anybody recommend a system to go for? We use a lot of Bio-Rad's protein gel systems, but don't know which one is best for 2D gels.

07 July 2020 6,327 3 View

Is there any open source code for learning in the field of data-driven robust optimization?

I can get a lot of theoretical knowledge in related fields, but I'm still confused about the implementation behind it. Unfortunately, I didn't find any examples on the Internet that I could...

29 March 2020 6,438 3 View

Why the large grain contains small grain inside ?

We currently recieved a sample from industry. It is high manganese steel (Hadfield steel) with a composition of Fe-12Mn-1.1C, after austenization treatment. We find that the grain size of this...

15 January 2020 6,624 1 View

How to read the intersection signal information in the Internet of Vehicles environment?

The existing method can be realized by short-distance traffic through the DSRC, or the signal light device can be connected to the roadside device RSU by wire. Even in the driverless field, the...

18 November 2019 6,706 1 View

Are there recommendations to buy some small mobile robots to test our multi-robot algorithms?

We would like to validate and test our multi-robot algorithms with some research-platformed mobile robot. Are there recommendations to buy some small mobile robots to test our multi-robot...

15 November 2019 5,772 3 View

How should the bone model be considered when screw drilling into bone finite element modeling?

For the establishment of bone model, besides considering the bone density, elastic modulus, poisson's ratio, yield stress and yield strain, should the damage caused by bone being cut by screws be...

09 November 2019 5,831 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Julio C. Morales

What is the tumor type? That will help in determining what media would help you most.

Xiuquan Luo

Ovarian cancer will be the first to test.

Cheng shi Shi

Hello! I would like to ask you how is your primary ovarian cancer tumor cell culture? Can you provide a specific protocol or experience for me to learn from? Thanks!