How can I be sure that my bone marrow cells are differentiated into bone marrow derived macrophages (day 7) at a gene expression level?

Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...

09 July 2020 1,742 3 View

Insects and COVID-19: a possible contaminant?

Dear colegues, What do you think about this? Can insects or others arthropods transmit the virus (directly or indirectly)?

19 April 2020 7,391 53 View

How can I identify and catalog the Rainforest fungus?

We have a big area of Rainforest where we can see a big variety of fungos growing around. I'd like to catalog them using a simple methodology. We do not have equipment for that despite a...

16 March 2020 9,034 3 View

I tried metawin unfortunately I couldn't get much help on how to code studies. Does anyone know a good free package for correlation meta analysis?

By the way I also need advice on which correlations to extract from factor studies ( exploratory, confirmatory, & SEM PLS) for meta analysis?

16 March 2020 7,217 3 View

Deep Euthetic Solvents, how to prepare?

Hi, everyone. I am starting to work with fractionation of Biomassa by Deep Euthetic Solvents . I came across a DES that is comprised of Lactic acid: Glucose:water (molar ratio 5:1:3), as in ref....

12 March 2020 3,474 3 View

Forests Pests: increased damage by climate change?

Dear researchers, what is your opinion about climate changes and forests pests? there is an increase or reduction of damage caused by insects and mites on trees?

29 February 2020 2,434 17 View

How to deal numerically with the discontinuities coming from a Butler-Volmer interface condition in a secondarry current distribution model??

Let us suppose that we want to compute the secondary current distribution of an electrolytic cell composed by anode, electrolyte and cathode. The governing equation in each subdomain isgiven by...

17 February 2020 7,913 3 View

How do I read the output files for a TDDFT (UV-Vis) job calculation (specially the graph itself)?

Hi everyone, so, I did some TDDFT calculations and have my .log files. I know I should be looking for the oscillator strength, the energies in eV and so on..., but my problem is this: How can I...

05 February 2020 7,783 3 View

Can one conduct a meta analysis on previous meta analyses?

When meta analyses on the same topic yield different results with no consensus, can one conduct a meta analysis on the Meta analytic studies?

19 December 2019 6,113 4 View

HOMO LUDENS: how do you use it in your academic research?

Homo Ludens is a book published in 1938 by Johan Huizinga. It discusses the importance of the play element of culture and society, and is a fundamental part of my PhD dissertation (work in...

11 November 2019 9,388 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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03 March 2021 5,360 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View