Hello community. I am interested in quantifying heterogeneity of a "noisy" cyan fluorescent protein transgene in a U-2 OS cell line. So far we've generated some nice flow cytometry data suggesting bimodal expression (similar to variegation). But these data do not satisfy my curiosity about the gene's behavior in time and space, as in a colony of dividing cells. The cells form pretty nice colonies on TC-treated plates seeded with very low concentrations of cells. However, the colonies are hard to look at over time because they are usually scattered all over the plate surface.
My question: Is there a commercially available TC vessel/ substrate that is printed with nicely arrayed "sticky" spots to trap cells and allow them to grow into colonies? I thought about spotting an non-TC-treated plate with poly-L-lysine, but I figured that others may have already developed more sophisticated solutions. My favorite example is this paper from T. Peterbauer et al, 2006: "Simple and versatile methods for the fabrication of arrays of live
mammalian cells" (https://pdfs.semanticscholar.org/0c35/fe12986af920e92e966e36fef15f7a737cc8.pdf)
Thank you for your help.
https://pdfs.semanticscholar.org/0c35/fe12986af920e92e966e36fef15f7a737cc8.pdf
Cytoo is the way to go. Lots of flexibility in substrate material, pattern shape, and vessel type (chips, plates, etc).
https://cytoo.com/
I've used their chips in the past and the product works well. A little bit pricey versus just patterning something yourself, but it's certainly doable.
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