Has anybody had any issues with Rotenone precipitating out in cell culture medium. I see brown precipitates forming in cell culture media after 1-2days in culture at doses above 30uM. If so, what can be done to avoid this?
I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...
01 June 2024 338 2 View
Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...
26 May 2024 2,151 6 View
I faced with a problem, and I think you can help me regarding this issue. I have sent one of my PCR poducts (ITS1-ITS4) to a company in order to sequence it. Indeed, I sent them various PCR...
01 February 2024 2,626 3 View
Applying Physical Geo Method in Finding Place to Build Bridge and applying Bases
09 December 2023 5,819 1 View
Geological hazard evaluation and monitoring (tools, methods, maps, etc.…)
09 December 2023 1,014 2 View
What is your solution regarding extra bands in ITS molecular markers? I am trying to use ITS1 and ITS4 markers in 15 plant species. Unfortunately, I only received extra bands instead of one after...
28 November 2023 9,305 4 View
I've recently faced a question that I couldn't solve on my own or with colleagues. I want to know what are PCR biases and how to handle them? Any answer in welcome.
20 June 2023 9,275 1 View
Hi all, I have two questions regarding taxonomy 1- how do you define a novel family? or even order? 2- if you have a strain that is not deposited in two culture collection, can you describe it...
20 June 2023 6,362 1 View
I want to make two solutions, one using 80% ethanol and the other one using distilled water.
17 May 2023 113 1 View
I have two morphologically similar species that differs molecularly only by 4 bases. Can I synonymmized them? If not, how many base differences can lead to this taxonomic revision? How do I deal...
10 April 2023 3,602 18 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View