Is a wall with constant pressure and constant inlet flow similar to an inlet boundary?

At an opening in turbulent flow, using wall boundary condition, with constant flow rate and constant pressure inlet, would be similar to an opening with inflow BC?

03 February 2021 2,390 5 View

Is it possible to estimate the most recent common ancester using o & 1 data? If yes would you please introduce a software?

Dear professors Respectfully, i know that sequences can help us to reveal the most common ancestor in phylogenetic studies. However, in genetic diversity studies using IRAP, SSR, markers etc, i...

13 November 2019 9,305 6 View

How has the absorbed solar pump power been calculated (Please explain in more details)?

During ray-tracing, the active medium is divided into a total of 18,000 zones. The path length in each zone is found. With this value and the effective absorption coefficient, the absorbed...

02 December 2018 1,216 1 View

How has the absorbed solar pump power been calculated (Please explain in more details)?

During ray-tracing, the active medium is divided into a total of 18,000 zones. The path length in each zone is found. With this value and the effective absorption coefficient, the absorbed power...

01 December 2018 4,673 1 View

How can I perform volume integration of product of E-field distributions in different frequencies in COMSOL?

My structure is periodic and I designed its unit cell in COMSOL. I used Electromagnetic waves,Frequency domain physics and Frequency domain study and swept on different frequencies. I want to...

05 February 2018 7,925 1 View

Identify DNA bands transfer from gel to the membrane(southern blotting)?

Hello Is there a way to detect transferring of DNA bands from gel to membrane in southern blotting without staining? Thanks

10 November 2017 7,804 3 View

Can anyone suggest the best method for measuring PON1 arylesterase activity in serum?

05 January 2015 3,060 1 View

Is there a possibility to see any green substance in extracted DNA?

I saw a green substances in potamogeton DNA. Does anybody know anything about this?

10 May 2014 5,702 4 View

What is the cause of green DNA in Potamogeton extracted DNA and how can I delete that?

Green DNA of Potamogeton and deleting the color.

05 May 2014 9,649 3 View

Hydrological test statistics

What is the best test statistic for gamma distribution?

01 January 1970 7,770 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View