I wish to compare the level of ppGpp in a Wt strain and mutant. I performed my protocol with following references: Lutz Wehmeir et al, 2001; Gonzales and Collier, 2014. My question: How to make a positive control of the stringent response knowing that the addition of Serine Hydroxymate (50microL of Stock50mg/mL) in my strains revealed nothing on TLC membrane (No detection of any spot)? Are there other runways using amino acids or something different? More details about my experiences/ Media: MOPS+Glu+17AA / Model organism: E. coli

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