Can anyone suggest me how to do this standardization curve?
I am thinking to either using a method where I set time points and at each time point, I read the OD600 along with doing serial dilution and plating on agars OR pick one time point and serially dilute it so I have several diluted bacteria (read OD600 each of them) then each of these goes to serial dilution and plating on agars.
If someone can suggest me other methods. I really appreciate it.
I want to refer to this curve whenever I need to know the concentration of the killed bacteria.
Sara Shahid
You can also make different concentrations of McFarland and draw a standard curve from their OD values.
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