How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

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23 February 2021 4,014 3 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

How do I dissolve a TPU (Texin RxT85A) in THF?

I want to make a 6% solution of Texin RxT85A in THF. I weighed the necessary amount of Texin resin and mixed at room temperature with THF with the help of a wooden stick, this did not work at all....

18 January 2021 9,080 3 View

Is there an alternative, more ecological solvent for shape memory polyurethane other than N, N-dimethylformamide (DMF) ?

As DMF creates problems for environmental aspects, we are looking for an alternative solvent for potential commercial end use applications of SMPU.

25 November 2020 8,728 3 View

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When producing a recombinant protein, harvested E. coli cells are normally refrigerated for 12-24 hours before homogenization to ensure protein inactivation due to foaming does not occur....

16 October 2020 7,373 0 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

What does over foaming correspond to in E.coli fed-batch fermentation?! How does foaming relate to cells metabolic status?

I'm currently experiencing overfoaming in my E.coli culture. My cells are used to produce a recombinant protein. The basic cell strain is BL-21 DE3. After changing my carbon source brand i am...

06 October 2020 3,946 3 View

What is the influence of the mold release agent on sound absorption properties of polyurethane foam ?

I'm an intern in a big company which makes flexible polyurehane foam for the automotive sector and I'm working on sound absorption properties of PU foam. I'm not chemist so I'm trying to improve...

23 September 2020 3,765 4 View

Which damage criteria should be used for nonlinear viscoelastic behavior?

Hello, I am building a material model for a nonlinear viscoelastic polymer in ABAQUS. For the beginning I just want to simulate an uniaxial tensile test. But now I want to know which damage...

13 September 2020 6,606 4 View