26 July 2018 1 6K Report

I used Poly-DL-ornithine hydrobromide to coating plating. Do you know how much I can store the diluted to 4 C?

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Is it possible to add multi-step analysis in ANSYS Mechanical APDL?

Hello everyone, I want to run a multi-step analysis in ANSYS Mechanical APDL. I have 83 load cases. The force is located at the same elements, the force direction is the same, the boundary...

26 March 2023 2,705 3 View

While using ToxTrac, sometimes the tracking process stops abruptly and the program shuts down before the process is completed. How to solve this?

Hello, I am using Toxtrac for the first time in order to detect the movements of mice while they are habituating in the open field box. Even though the program is fairly easy to use, I am stilll...

10 February 2022 2,842 3 View

What software is capable of opening a file with the extension ".cad" ?

It seems like quick cad was the software but had been discontinued. I tried with other Autodesk software such as fusion 360, AutoCAD. I also tried with QCAD. Please let me know if you know of any...

22 February 2021 10,003 3 View

Can anyone recommend an electronic Lab Notebook software, which is not Cloud based?

Hi everybody! We would like to switch to electronic lab books, but all software that looks interesting is cloud based. We would like to avoid a cloud based version because we have concerns that...

04 October 2016 9,751 3 View

How do I analyze expression data with the IPA Software?

Hello IPA users! I am currently testing the IPA Software with my expression data and wonder if I follow the right strategy. I am working on a case with chronic lymphocytic leukemia (CLL) and a...

28 April 2015 8,588 1 View

Can you suggest any treebank generator?

I 'm interested in the syntactic annotation of English texts. Thanks.

19 January 2015 3,436 3 View

Educational paradigms - what are your thoughts?

Barr and Tagg, 1995 provided an informative comparison between 'The Instruction Paradigm' and 'The Learning Paradigm' in relation to mission and purposes of each (e.g. provide/deliver instruction...

03 March 2014 1,882 10 View

What do you think the consequences of a 'drop in motivation' in employees will be?

Amir and Lobel (2014) state, 'The drop in Motivation and results may be more damaging to companies than the actual loss of the employees'. Amir and Lobel in their Harvard Business Review...

06 February 2014 1,509 19 View

Do you think performing psychometric tests prior to interviewing makes for a more efficient hiring process?

In their Harvard Business Review article, Bateson, Wirtz, Burke and Vaughan (2014) say: 'When hiring, first test, and then interview In their interesting short article published in the Harvard...

06 February 2014 3,094 39 View

Guptara (2010) outlined seven steps to creating the right kind of globalization. In your opinion, can we implement these?

Outlining four key issues in our globalizing economy (unprecedented wealth, unprecedented poverty, ecological challenges and political and economic volatility), Professor Guptara goes on to...

29 January 2014 4,951 24 View

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Adhesion strength of coating?

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I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

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Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

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PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

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Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

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What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

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