Plz help me in designing the primers used to quantify the gene expression in heavy oil biodegrading Paenibacillus ehimensis,B.halodurans,B.firmus,B.subtilis and B. licheniformis for ddPCR?? What will be the reference genes used ? is it 16 S gene??
I'm currently detecting the AlkB gene in Bacillus strains potent of biodegradation. I found in an article about the organisms mentioned as the positive controls for PCR using the same primer sets...
09 October 2014 9,493 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I want to know more about petroleum deposits in Iran.
02 August 2024 8,725 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View