Please give me your experience in that
aim in a total need for your advice
I think it is something that varies from lab to lab. You might have to standardize the conditions yourself. Do you have anything specific to ask like transfer time, voltage, antibody concentration etc. Then may be I might give a helpful answer.
Best wishes
in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?
08 September 2019 6,957 4 View
if i can able to detect the transcript cd36 from cells but not from brain tissue .'.expected to have it'. ciuld it have more secondary conformational chnges than from cells?..just IDK
08 September 2019 5,637 5 View
Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?
02 March 2019 7,230 3 View
i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...
11 December 2018 1,983 2 View
10 mins at 60 degrees vs 5 min at 95 degrees
10 November 2018 714 7 View
iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...
10 November 2018 4,157 1 View
brain tissue reverse transcription..
10 November 2018 2,274 0 View
i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...
10 November 2018 5,897 6 View
i mean i will do qpcr i will multiplex my housekeeping taqman gene (pl) vic dye with another taqman gene and will do the rest in singleplex format ? can i compare other genes expression to...
10 November 2018 2,868 3 View
modified Y maze , would be glad if you posted advices /papers
09 October 2018 7,257 0 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...
01 March 2021 2,294 3 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...
23 February 2021 5,503 1 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...
18 February 2021 190 3 View