In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Please Guide me on this how to solve this issue ?

I have submitted a paper in a journal and the reviewers reply to me with this. Can anyone guide me on this how to solve it Thanks in advance Figure 4: (i) the image presented in panel (a) shows...

15 December 2020 636 6 View

How to compare two fMRI sessions using SPM12 (inter-sessions differences) ?

I'm performing an fMRI analysis for my master thesis using SPM and I've never worked with fMRI data before. I've succesuffy perform first level analysis but came to an obstacle. Study design...

05 December 2020 6,956 2 View

How to reduce pH of a liquid solution?

Hello, Everyone! I am working on TiO2 composites and our prepared materials solution has a pH of 13 which is totally basic, and the process of pH value decrement is too slow could any please...

11 November 2020 7,467 10 View

Has anyone observed delamination of pyrolysed SU-8 on SiO2 coated Silica substrates when the pyrolysis is performed outside cleanroom?

We have a quartz tube furnace (diameter 80 mm) outside the cleanroom and when tried to pyrolyse the SU-8 on SiO2 coated Si substrates at 900 C in nitrogen atmosphere, the electrodes looks fine....

08 November 2020 4,357 2 View

How to calculate element percentage from XRD analysis for mineral identification?

I have carried out a XRD analysis to determine mineral identification. I used Origin 8 program in phase identification. I couldn't carry out XRF. Is it possible and reliable to obtain different...

13 October 2020 9,228 8 View

Help to produce graph in ORIGIN PRO?

Hi, I want to produce a graph in Origin, comparing two groups of data, in the form of scatter points, where the mean and SE bars are included. See attached photo for reference. It is simple to...

25 September 2020 9,756 1 View

Can be episomal plasmid vector amplified in mitotic cells?

Can be episomal plasmid vector amplified in mitotic cells without host- suitable ORI such as SV40 in mammalian cell that express sv40 large T antigen?

24 September 2020 1,570 4 View

I have got the following photo luminescence spectra data of AU-PD tio2 film for diffrent depotion rates ,can some one help me to analyse it broadly?

i have got the following photo luminescence spectra data of AU-PD tio2 film for diffrent depotion rates ,can some one help me to analyse it broadly? can we discuss it ??

02 September 2020 3,260 6 View

SU-8 air bubble problem?

23 August 2020 9,193 7 View