We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types).
I need your recommendation on how to determine (quantitatively) Src inhibition. Note that ATP competitive inhibitor assays do not serve our purpose.
Thank you
Hi Moustafa,
Maybe you can run a real-time functional global kinase activity array from PamGene with your inhibitor in an ex-vivo inhibitor profiling assay. These assays are more sensitive (1-5 ug proteins only, 50K-100K cells), robust, reproducible (
Dasatinib is an Src inhibitor. May be it would help you reading the papers on molecular studies on dasatinib. You will probably find among them the best method for Src inhibition quantification.
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