Tushar Tomar

6 Questions 47 Answers 0 Followers

Questions related from Tushar Tomar

How to culture primary patient-derived breast cancer cells?

I am culturing cells from primary patient tumor in 2D using normal 10% FCS containing medium and 3D (mammosphere) using Mamocult medium. However they are in general slow growing. Any advise or...

27 July 2016 639 2 View

Can anyone suggest an alternative method over western blot if no good antibody is available for a gene product i.e. the protein?

I have transfected tumor cells with pcDNA for a gene of interest. I have tried 5 antibodies for the same gene from different providers, but none of them worked nicely so far. I have mRNA...

03 March 2015 7,297 19 View

How can you find equally methylated regions in different samples using Infinium 450K array in R?

I have methylation data from Infinium 450K and I found out differentially methylated regions. Is there a way to find the equally methylated regions as well, particularly in R. Recommendation for...

07 November 2014 2,264 3 View

Can anyone share any experience for siRNA treatment for an in-vivo experiment on orthotropic cancer mouse model?

Would anyone like to share his/her experience on siRNA treatment for in-vivo experiment of cancer mouse model particularly for peritoneal cavity? I would like to know how frequent (daily or every...

14 March 2014 8,837 2 View

How can we design customised siRNA targeted against our gene of interest?

I have little experience in siRNA designing.... 1) I looked at literature where some time only one sequence is mentioned and sometime sense and antisense sequence...Further sometimes ended with TT...

22 February 2014 7,375 8 View

Which is the best and easiest R software package for infinium methylation 450K?

I am recently going to work on infinium methylation 450K array. I would like to analyze them using R for findign DMRs in my two groups. Can you suggest me which R software package would be easy...

14 February 2014 6,735 3 View