Could you get me this report, please?

03 April 2018 7,186 1 View

Hi Please as you know that neutrophils are the only cells that can express Ly6G. So can I ask which antibody is specific for this marker?

actually, some references mentioned to use Gr-1, however, I think it would be inappropriate to use Gr-1 antibody as it was reported before that it contains antibody for both Ly6C and Ly6G whereas...

02 March 2018 8,397 1 View

The most common types of bacterial contamination that affecting the growth of anadara granosa or Blood Cockles?

Dear all Professors, Researchers, and Colleagues, I need your help by list ''What are the frequent types of bacterial contamination that affecting the growth of anadara granosa or Blood Cockles...

10 November 2017 1,943 0 View

Why assigning the shell section by selecting middle surface, top surface, or bottom surface to represent the reference surface makes difference?

I'm modeling thin-walled columns using shell element in ABAQUS. I'm using different ways to shape the geometry to end up with the same final configuration whatever the way is. I noticed that when...

09 October 2017 1,061 0 View

Do I need to do Symmetry for beam element?

Dear all, I'm trying to reduce the computational time for a model which contains circular shell element and beam element (with pipe profile) in the top ( see the attached pics). Do I need to do...

05 June 2017 8,630 3 View

What are the parameters should I input for this UMAT code to get results similar to ABAQUS GUI?

I just wrote the Isotropic Hardening rule Fortran code (attached below), which is similar to what's available in ABAQUS library, if you have experienced with this code before, what are the...

31 December 2016 5,980 3 View

What's the procedure to get the original written data from a figure in published paper?

I'm wondering if there is a way to get the original data for some figures in published research papers? I've tried to contact the authors, but no responses come back, could I get this data from...

11 December 2016 9,775 2 View

How can I export or import part, sketches or assemblies from ABAQUS?

I want to use the same geometry that I drew it in ABAQUS and import again in a different ABAQUS model (not copying). I'm trying with File-export to save it, but when I'm gonna import it again, it...

09 October 2016 6,450 4 View

Link ABAQUS 6.14 with intel parallel studio XE 2016?

09 October 2016 2,061 5 View

Thin-walled column under constant axial and lateral cyclic loading?

09 October 2016 5,724 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View