Please it would be much appreciated if there is anyone knows how can we use the imagej to measure the size of cells

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Could you get me this report, please?

Public work research institute of Japan. 1997–2000. Report of cooperative research on limit state seismic design for bridge piers, I-VIII and summary.

03 April 2018 7,248 1 View

Hi Please as you know that neutrophils are the only cells that can express Ly6G. So can I ask which antibody is specific for this marker?

actually, some references mentioned to use Gr-1, however, I think it would be inappropriate to use Gr-1 antibody as it was reported before that it contains antibody for both Ly6C and Ly6G whereas...

02 March 2018 8,474 1 View

The most common types of bacterial contamination that affecting the growth of anadara granosa or Blood Cockles?

Dear all Professors, Researchers, and Colleagues, I need your help by list ''What are the frequent types of bacterial contamination that affecting the growth of anadara granosa or Blood Cockles...

10 November 2017 2,040 0 View

Why assigning the shell section by selecting middle surface, top surface, or bottom surface to represent the reference surface makes difference?

I'm modeling thin-walled columns using shell element in ABAQUS. I'm using different ways to shape the geometry to end up with the same final configuration whatever the way is. I noticed that when...

09 October 2017 1,146 0 View

Do I need to do Symmetry for beam element?

Dear all, I'm trying to reduce the computational time for a model which contains circular shell element and beam element (with pipe profile) in the top ( see the attached pics). Do I need to do...

05 June 2017 8,704 3 View

What are the parameters should I input for this UMAT code to get results similar to ABAQUS GUI?

I just wrote the Isotropic Hardening rule Fortran code (attached below), which is similar to what's available in ABAQUS library, if you have experienced with this code before, what are the...

31 December 2016 6,059 3 View

What's the procedure to get the original written data from a figure in published paper?

I'm wondering if there is a way to get the original data for some figures in published research papers? I've tried to contact the authors, but no responses come back, could I get this data from...

11 December 2016 9,837 2 View

How can I export or import part, sketches or assemblies from ABAQUS?

I want to use the same geometry that I drew it in ABAQUS and import again in a different ABAQUS model (not copying). I'm trying with File-export to save it, but when I'm gonna import it again, it...

09 October 2016 6,592 4 View

Link ABAQUS 6.14 with intel parallel studio XE 2016?

Hi all, I'm trying to Link ABAQUS 6.14 with intel parallel studio XE 2016, and Microsoft visual studio 2013 professional, could you please tell how I can do that? or what kind of Intel parallel...

09 October 2016 2,143 5 View

Thin-walled column under constant axial and lateral cyclic loading?

Hi, I'm modeling thin-walled column under axial loading and horizontal displacement as shown in attachment 1 using displacement control (Boundary conditions). I decide and input the horizontal...

09 October 2016 5,799 7 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How does one derive the standard deviation of a scale?

Dear all, I am working on analyzing data from a survey on student satisfaction. The survey contains items with a 7-point Likert response format that produce 12 scales related to different areas...

05 August 2024 2,141 4 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View