Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Has anyone tried performing a transient transfection on a cell line that has been stably infected with a lentivirus?

Hello, I was having difficulty in infecting a cell with a gene of interest. Say, for instance, I was able to introduce a CMV on the cell, but I was not successful in introducing my target gene. I...

25 September 2019 2,412 3 View

Mouse Cell Line STR Profiles?

Does anyone have STR profiles of mouse cell lines from the following strains: C57BL/6 129 FVBN BALB/c Swiss black Which company did you use to authenticate the cells?

07 April 2019 4,519 2 View

Can 2 way ANOVA be used in nonexperimantel research?

We will be studying about the role of spirituality and gender ( 2 IVs) on coping effectiveness (DV) and we shall be using standardized scales in measuring. Can 2 way ANOVA be used in our analysis?...

09 November 2017 8,917 4 View

Any information or studies about Wild Sampaguita (Jasminum bifarium) ?

My study for my undergraduate thesis is Phytochemical Evaluation, Antibacterial Activity and Angiogenic Property of Wild Sampaguita (Jasminum bifarium). I am using leaves for my study and I need...

25 March 2017 3,487 3 View

Qubit measurements of gDNA increasing over time?

Dear all, I am facing Subit issues, for mosquito genomic DNA measurements (for gDNA library preparation for NGS). The same sample was measured by 3 different people (in december 2015, august 2016,...

26 February 2017 2,726 5 View

Why don't we have bacteria growth in our liquid LB ?

We are facing a problem in different groups in our institute: when we grow E. coli (DH5a) overnight in liquid LB, we have: - no growth at all - sometimes, some tubes show cell growth, some...

20 February 2017 2,314 12 View

Does anyone have troubleshooting experience for low on-target exome enrichment (Nextera)?

I have prepared 4 libraries (Nextera exome 37mb), first with 12 samples and prepared 3 months before the next 3 (of 24 samples). The first had low enrichment (40% of reads on-target), the second...

30 June 2015 3,324 1 View

Can I use Agencourt Ampure XP for genomic DNA purification?

I have genomic DNA eluted in TE buffer, and am planning to sequence them. Unfortunately, EDTA should be avoided for Nextera library preparation. I am thus planning to purify my gDNA again, using...

19 March 2015 4,615 22 View

How common is five-fold symmetry among plants and animals?

06 July 2014 6,460 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View