Is it safe to remove performic acid from protein via freeze drying?

I use solution of performic acid (30% hydrogen peroxide and 80% formic acid mixed 1:9) to solubilize a protein. Is it safe to separate the performic acid from the protein via freeze drying? Should...

25 November 2020 8,045 3 View

Dear all , I am wondering to know about 18S RNA amplification cycle. Which cycle is the starting point of 18S RNA amplification in qRT-PCR?

02 October 2017 858 3 View

Does anyone have any suggestions on to incorporate DNA in my Cos-1 cell line?

I'm trying to introduce a plasmid encoding for a fusion protein calreticulin-RFP but for now I have no positive expression of the plasmid info. The modification was done a week ago. Any...

18 February 2016 4,054 5 View

Do someone know any graphic or cartographic method to link land-use and mobility processes or results ?

Exploratory data analysis (Tukley, 1977) is a solution. But did someone experience any other solution to indentify the link beetwen land use changes and urban mobility ?

03 February 2016 9,874 3 View

Which is the best procedure for hybridizing two DNA strands in solution?

I have two DNA strands (one has 39 base pairs and the other 10 base pairs, both being complementary) and I want them to hybridize together in solution. I usually mix the two strands (final volum...

18 November 2014 1,008 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View