Please, does anybody have any culture of E.coli DB3.1 or any other ccdB survival strain for plasmid cloning they could share?

More Antoni Gandia's questions See All

How to reach the people of the International Journal of Economics and Finance Studies to fix errors in publication???

I have published an article in the International Journal of Economics and Finance Studies, and the editing of the final article is wrong. They have missed spelt the authors' surnames and...

06 June 2023 2,400 2 View

Is it safe to remove performic acid from protein via freeze drying?

I use solution of performic acid (30% hydrogen peroxide and 80% formic acid mixed 1:9) to solubilize a protein. Is it safe to separate the performic acid from the protein via freeze drying? Should...

25 November 2020 8,247 3 View

Thermophysical properties of mettalic gases?

Hi everyone, my colleagues and I are currently developing a deep penetration laser beam welding model. Since in such a model the vapor phase is included, we need/want to consider very high...

30 March 2020 4,903 9 View

This is a call for papers for a special issue on Mineral Deposits of Latin America and the Caribbean. Anyone interested?

Special issue on Mineral Deposits of Latin America and the Caribbean (Boletín de la Sociedad Geológica Mexicana) Heads up! We are intending to publish a special issue on Mineral Deposits of...

26 August 2018 2,664 23 View

Dear all , I am wondering to know about 18S RNA amplification cycle. Which cycle is the starting point of 18S RNA amplification in qRT-PCR?

I would like to use 18S RNA as a house keeping gene in real time PCR. Plz share your best experience.

02 October 2017 944 3 View

How to read MIT-BIH ECG annotation files?

After reading MITDB ECG annotation files using the Matlab wrapper for WFDB, I get five columns. The first is the time interval and the third seems to be the beat type. I assume fourth and fifth...

10 June 2017 3,138 8 View

Any insight about possible stratified brines in the epithermal environment?

Stratified brines have been detected or discussed in various hydrothermal environments, particularly on the seafloor, the Salton Sea case, etc., but is anyone aware of the simultaneous occurrence...

09 March 2017 9,668 1 View

Any evidence for hydrothermal activity in epithermal deposits terminated by volcanism?

We all know that reactivated volcanism may dismantle convection cells of shallow hydrothermal activity (which is a very reasonable idea), but can anyone provide compelling evidence, geochronoligic...

11 July 2016 8,018 12 View

Does anyone have any suggestions on to incorporate DNA in my Cos-1 cell line?

I'm trying to introduce a plasmid encoding for a fusion protein calreticulin-RFP but for now I have no positive expression of the plasmid info. The modification was done a week ago. Any...

18 February 2016 4,156 5 View

Do someone know any graphic or cartographic method to link land-use and mobility processes or results ?

Exploratory data analysis (Tukley, 1977) is a solution. But did someone experience any other solution to indentify the link beetwen land use changes and urban mobility ?

03 February 2016 10,012 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View