How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Is there no general protocol for vaccination study?

Hi, I searched vaccination study to know which MLD50 is suitable for influenza virus study in pubmed. But why every vaccination and challenge conditions are different? Is there no guideline or...

16 January 2021 7,984 3 View

Hi, I did a Plasmid DNA isolation and got the concentration of 0.0414ug and colleague got 0.1391ug, how do I compare the two results?

Our volume that contain 0.1ug of our sample plasmid DNA were 2.4154ul and 0.7189ul respectively. How should I do the comparison, please

10 November 2020 9,777 9 View

How can I generated Simulated/Predicted Effects from an MRQAP model?

I want to generate some nice prediction plots from my MRQAP model. I've laid out my process below, and would be very grateful to get anyone's insight, as I'm not seeing much written about this...

05 November 2020 1,215 2 View

Best Flow Cytometry Rat Spleen processing for Macrophages?

I'm trying to design a protocol to process rat spleens to maximize macrophage yield for flow cytometry. Currently I plunge my spleens through a filter and incubate with lysis buffer before...

29 October 2020 5,671 2 View

Does anyone know of any sets of surface colour spectral reflectance curves in the visible light region (i.e. from 400nm to 700nm) available online?

I think there are physical limitations on the spectral reflectance curve that can be achieved by a physical object. The small number spectral reflectance curves I have found online seem to share...

10 September 2020 5,357 5 View

Is it OK culture E.coli at RT for overnight?

For plasmid DNA isolation, E.coli was incubated last night. But incubator doesn't work and I just knew it this morning. The incubator fixed now. Is it okay for my experiment?

28 April 2020 2,931 10 View

Is there a way to batch-convert mass spectrometry cdf files to mzML files?

I have a set of roughly one hundred cdf files from a mass spectrometry experiment. I would like to analyse them using MetaboAnalystR, which requires mzML files as input. I know I can convert each...

28 March 2020 1,794 6 View

Is it possible to predict the intermetallic compounds/phases that will be formed in an alloy (i.e. maraging stee) only from its chem. composition?

I am trying to determine an intermediate phase/compounds that will be formed and its fraction (if possible) as well as their sequence (If more than 1 phase) from its chemical composition...

24 March 2020 1,889 4 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

How to report Fishers Exact test from SPSS in a report properly?

I am wondering how do I write the data I have collected from my fishers exact test into words so that it backs up my work? the exact sig.(2-sided) was 0.033, meaning it is significant because its...

17 February 2021 3,441 7 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

How to test the equality of two proportions with multiple samplings?

02 February 2021 9,574 5 View

Western blot transfer problem - what could cause it?

Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...

25 January 2021 4,228 6 View