How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Is there no general protocol for vaccination study?

Hi, I searched vaccination study to know which MLD50 is suitable for influenza virus study in pubmed. But why every vaccination and challenge conditions are different? Is there no guideline or...

16 January 2021 7,984 3 View

How can I generated Simulated/Predicted Effects from an MRQAP model?

I want to generate some nice prediction plots from my MRQAP model. I've laid out my process below, and would be very grateful to get anyone's insight, as I'm not seeing much written about this...

05 November 2020 1,215 2 View

Best Flow Cytometry Rat Spleen processing for Macrophages?

I'm trying to design a protocol to process rat spleens to maximize macrophage yield for flow cytometry. Currently I plunge my spleens through a filter and incubate with lysis buffer before...

29 October 2020 5,671 2 View

Does anyone know of any sets of surface colour spectral reflectance curves in the visible light region (i.e. from 400nm to 700nm) available online?

I think there are physical limitations on the spectral reflectance curve that can be achieved by a physical object. The small number spectral reflectance curves I have found online seem to share...

10 September 2020 5,357 5 View

Is it OK culture E.coli at RT for overnight?

For plasmid DNA isolation, E.coli was incubated last night. But incubator doesn't work and I just knew it this morning. The incubator fixed now. Is it okay for my experiment?

28 April 2020 2,931 10 View

Is there a way to batch-convert mass spectrometry cdf files to mzML files?

I have a set of roughly one hundred cdf files from a mass spectrometry experiment. I would like to analyse them using MetaboAnalystR, which requires mzML files as input. I know I can convert each...

28 March 2020 1,794 6 View

Is it possible to predict the intermetallic compounds/phases that will be formed in an alloy (i.e. maraging stee) only from its chem. composition?

I am trying to determine an intermediate phase/compounds that will be formed and its fraction (if possible) as well as their sequence (If more than 1 phase) from its chemical composition...

24 March 2020 1,889 4 View

Is there a free Raman Spectra database that I can search for a set of given peaks?

Is there a free Raman Spectra database that I can search for a set of given peaks? Thanks in advance for pointing the way.

22 December 2019 4,237 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View