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Questions related from Gillian Kay
We were making up the buffers for our Promega PureYield plasmid midi-prepkit and accidentally added 315ml of isopranolol to the endotoxin wash buffer instead of 210ml. Do you think we can use it?
05 December 2023 9,281 1 View
I used the Flp-InTM T-RExTM System to stably transfect 293T cells so that my gene of interest is inducibly expressed by tetracycline. I want to transiently transfect with a luciferase reporter...
02 December 2021 4,531 1 View
One of our students accidentally used most of the proteinase K solution that came with BioRad's Singleshot cell lysis kit. There is no concentration written on the tube or in the protocol of the...
16 December 2020 8,141 3 View
From Bio-Rad's user guide (see below) it is not clear to me what is the best activation time to use. It states that 1 min gives optimal results for WB followed by immunodetection, but that 5 mins...
26 March 2019 1,684 4 View
I have transformed dh5alpha cells with pLenti CMV GFP Puro (658-5) constructs (results of Gibson assembly) and while I have colonies on my ampicillin plates, they do not grow in broth (16h at 37)....
07 January 2019 6,144 3 View
