Gillian Kay

3 Questions 4 Answers 0 Followers

Questions related from Gillian Kay

Why do my stably transfected cells die when I transiently transfect them?

I used the Flp-InTM T-RExTM System to stably transfect 293T cells so that my gene of interest is inducibly expressed by tetracycline. I want to transiently transfect with a luciferase reporter...

02 December 2021 4,517 1 View

What is the ideal activation time for stain-free gels?

From Bio-Rad's user guide (see below) it is not clear to me what is the best activation time to use. It states that 1 min gives optimal results for WB followed by immunodetection, but that 5 mins...

26 March 2019 1,656 4 View

Why won't my transformed dh5alpha colonies grow in LB amp?

I have transformed dh5alpha cells with pLenti CMV GFP Puro (658-5) constructs (results of Gibson assembly) and while I have colonies on my ampicillin plates, they do not grow in broth (16h at 37)....

07 January 2019 6,135 3 View