Plasmatic proteins solvent?

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How to upload a sequence or create an account in Trichokey?

I need to upload a sequence of a sequenced Trichoderma and I don´t know how.

20 May 2024 6,685 2 View

Colony PCR for genera confirmation?

Hi everyone, I would like to ask for the scientific community's help about colony PCR for generation confirmation. I have isolated bacteria from mice faeces and, after a first characterisation...

24 March 2024 6,347 2 View

Good day colleagues/Seniors. How can one incorporate an armed conflict environment in the environmental uncertainty scale?Any literature to back that?

Urgent response needed

16 January 2024 6,577 0 View

How can I quantify Dengue Virus serotype 2?

Hi, I'm working with viral cultures, specifically with Dengue virus serotype 2. I need to quantify it by a plaque assay or TCID50. Does anyone have a protocol or has worked with this serotype...

10 December 2023 2,364 0 View

What are some design ideas for annealing Si samples (mounting adhesively or mechanically and heating up to 600°C)?

I need to anneal mm-scale samples made on Si substrates up to 600°C. To temporarily mount them, I've seen things like high temperature epoxies, tapes, and pastes as well as mechanical mounting...

10 November 2023 4,327 3 View

How to make cholesterol soluble in water?

Good morning, I want to dissolve cholesterol in Krebs-Henseleit buffer in tissue organ bath chamber where I place tracheal tissue. Krebs-H. buffer is buffer composed of water and salts similar to...

18 September 2023 4,774 0 View

Are there any tools for Gene ontology/Enrichment for Bacteria with unique locus tags and missing gene symbols (ID)?

I have a set of RNA data, 2 strains and three conditions. I did the mapping and Deseq analysis in geneious using our genome. But I ran into a problem that I'm struggling to resolve. I'm able to...

22 August 2023 7,575 1 View

Could someone please provide the procedure for trained Innate Immunity?

22 June 2023 9,337 3 View

SK-MEL-103 STR profile?

Hi all. Do you know the STR profile of SK-MEL-103 melanoma cell line? I guess it is not available from ATCC... Thank you.

16 May 2023 3,956 1 View

Is it possible to compare groups with different sample sizes while performing 2-way PERMANOVA?

I am dealing with unbalanced designs - comparing groups with different sample sizes, however I'm not sure I cancompare groups with different sample sizes while performing 2-way PERMANOVA.

30 January 2023 7,358 4 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View