I used protein precipitation during compound optimization for lc-ms/ms analysis. However, my blank plasma shows me a giant peak intensity for the specific analyte transitions. This is strange because most of the analyte is of the column when I run a solvent blank. Yet the plasma blank shows me an intensity of 2.5 e5 and the retention time is different from that of the analyte. The analyte appears a 0.88 when running in neat solvent but when I run blank plasma, the intensity appears at 1.7 min. Please, can anybody help me find a solution to this issue if you may have encountered it before?
Post spike your analyte with blank plasma to make sure retention time of your analyte. If you see 2 peaks don't care about 2nd one. just put enough run time to wash it out
Dear Mr. Moodley
It seem thar your system is contamined. Different parts of the system can be replaced, or cleaned. If your MS system is a quadrupole it is possible to be cleaned, however it need to be done by an mass spctrometer specialist.
Best regards
Please go to this link :
ccc.chem.pitt.edu/wipf/Web/LCMS%20trouble%20shooting.pdf
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