Can anyone help me with Lugols staining Pigeon pea seeds?

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How do I separate methylglyoxal (pyruvaldehyde) and pyruvic acid using RP-HPLC?

Using a Restek Roc C18 5um column and a mobile phase composed of H3PO4/NaH2PO4 buffer aqueous solution (0.1 M NaH2PO4 acidified by H3PO4 to pH +/-2) and acetonitrile (v:v = 9:1) [flow rate of 0.6...

06 June 2023 1,870 8 View

How long should we stress in vitro sugarcane plantlets?

Good day all I would like some clarity concerning stressing sugarcane in vitro plantlets using ABA. I have done some reading and see that the time for stressing varies from hours to days. I...

18 May 2023 1,013 0 View

Can anyone possibly assist me with Agrobacterium transformation (LBA4404) of Tobacco ?

Good day all I am having difficulty with my agrobacterium transformations with regard to agro overgrowth after co-cultivation. I am currently using 100 mg/L timentin along with 400 mg/L...

19 April 2023 1,079 7 View

Why is a powder of Mn(NO3)2/SiO2 turning black in colour after oven drying at 90°C?

5 wt.% of Mn(NO3)2.4H2O was used as a precursor for the wet impregnation synthesis of MnOx/SiO2. After dehydration at 70 °C, a white paste was obtained and thereafter dried in an oven overnight at...

21 November 2022 8,900 3 View

Is it possible with CFD to model coal particles with different char combustion reactivities?

Hello Everyone, In my study I would like to perform CFD pulverised coal combustion simulations and also include maceral effects on char combustion. Please advise if it is possible in CFD...

05 May 2021 9,165 0 View

Plasma blank shows me a high intensity for my analyte peak even though the analyte was completely washed off the column. What is the reason for this?

I used protein precipitation during compound optimization for lc-ms/ms analysis. However, my blank plasma shows me a giant peak intensity for the specific analyte transitions. This is strange...

14 May 2020 7,287 3 View

Why is there an absence of peak/s in the NH3-TPD analysis of silica ?

The NH3-TPD of commercial silica (S.A= 223m2/g) has been run on a Micromeritics 2900 AutoChem II Chemisorption Analyser to determine total acidity. However, there was no peak observed post-run....

29 April 2020 2,363 3 View

What technique could be used to separate coal particles into small density classes e.g. interval 0.01 g/cm?

Hello, I am attempting to separate out the main microlithotype particles found in coal. The requirement is that the concentrated fraction needs to contain at least 80% (by mass) of a certain...

12 January 2020 348 1 View

Can anyone identify the weed species in the attachment?

It is likely an arable weed, growing among tomato field crops in the northern KwaZulu Natal province of South Africa. This weed was found to be infected with a strain of Tomato torrado virus...

08 August 2019 10,028 9 View

Does anyone know an antibody for detecting via flow cytometry MS2-p65-HSF1?

MS2-p65-HSF1 is a transcriptional activation domain. By binding to aptamers from sgRNA2.0 (see Konermann 2015, Nature) you can target it with dCas9 to regulate expression of a gene of...

21 February 2017 7,662 0 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to convert protein percentage into kg/ha?

I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.

11 July 2024 9,045 4 View

How can I determine the percentage of cell viability of cells seeded in 96 well plate ?

I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...

10 July 2024 7,145 2 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

How to transfer a full grown cell monolayer to another plate?

Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...

30 June 2024 9,300 0 View

Andrew Paul McKenzie Pegman

You can use the iodine starch reaction method outlined in the attached paper :)

Buren Logan Moodley

@Andrew Paul McKenzie thank you for your reply! I will attempt this method and let you know how it goes.

Sanjoy Kumar Bordolui

Check it.

https://www.dalynn.com/dyn/ck_assets/files/tech/SL95.pdf

Check

http://www.tulipgroup.com/MicroExpress/Accumix/PackInsert/Stains/207120410100_Lugols_Iodine.pdf