Plama/serum albumin depletion protocols for LFQ prteomics experiment?

Muhammad Alsherbiny @Muhammad-Alsherbiny

17 August 2022 0 985 Report

I am planning an LCMS based differential proteomics experiment of plasma samples. Is the albumin/Ig depletion will significantly enhance the downstream analysis in terms of the number of identified and quantified protein? Also, I wonder if there will be a reccomended in house protocol or even commercial kits for particularly albumin depletion which roughly presents ~50-60% of total proteins.

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