MANIGANDAN KRISHNAN

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Can anyone explain which buffer is suitable to extract proteins (both cytosolic and membrane) from colon tissues?

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How can I choose a secondary antibody for the antimouse monoclonal raise in humans?

Kindly suggest me which the selection of secondary antibody for Cox-2 primary antibody (a mouse monoclonal antibody raised against amino acids 580-598 of Cox-2 of human origin).

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