I have used HCl from bottle (36% stock, not expired, but a little orange) to prepare Tris-HCl pH 7.5 and the solution is a little pink; is that caused by the formation of magnesium chloride? Can I use the solution or should discard it?
A431 cells observed with an Olympus BX-51, 100x magnification. I noticed aggregates indicated by arrows, randomly diffuse in the cytoplasm; someone is visible also in the nucleus.I can not tell if...
31 December 2016 5,728 9 View
I have performed a cycloheximide treatment on HEK 293 cells (10 ug/ml CHX for 1 h, no FBS in the medium) and then I've performed a confocal immunofluorescence protocol. Nuclei are stained with...
03 April 2016 1,142 4 View
I've read some papers about this protein and its roles, but I'm not sure if it is expressed also in epithelial cells. Someone could help me?
31 December 2015 9,369 0 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...
18 July 2024 1,390 2 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...
12 July 2024 2,768 1 View