A431 cells observed with an Olympus BX-51, 100x magnification.
I noticed aggregates indicated by arrows, randomly diffuse in the cytoplasm; someone is visible also in the nucleus.
I can not tell if they are aggregates or some kind of contamination.
I have never observed such kind of inclusion body in A431 cells. In my opinion, the contamination is the most likely. How about the doubling time of the cells?
DAPI staining pattern confirms the "diagnosis" of mycoplasma contamination.
Dear all, my A431 cells are of course under anti-myco treatment (ciprofloxacin and minocyclin). My DAPI staining after treatments is negative for mycoplasma, whereas definitely positive prior treatments. This is the confirm that I have to carry on myco treatments and perform PCR besides DAPI staining for better detection of contamination levels.
Thank you!
Are You sure those are the correct cells? https://www.lgcstandards-atcc.org/~/media/Attachments/0/0/2/F/1819.ashx
Yes they are. They are seeded on poly-D-lysine pre-treated coverslips, for this reason they may appear different in shape from ATCC catalogue.
What kind of contrast is it?
For me it could be just vacuoles, antibiotics can be bit toxic. IMHO DAPI testing is good to confirm infection but not enough to confirm its absence. I read somewhere that detection limit is 100 mycoplasmas per cell.
I agree with you as regards DAPI (as soon as possible I will do a PCR to evaluate treatment efficiency); as regards vacuoles (or of course simple lipid droplets) I not completely agree with you. What strikes me the most is the one inside the nucleus...
I must say I see just one photo so please forgive me ;-) I do not know on which level the spot is, below, over or inside the nucleus.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology