I am wondering if it is possible to use pillar array columns for separations other than in the reversed-phase mode? I have found a paper explaining the difficulty of coating the pillar surface with C18 being the small interpillar spaces, and because of the adsorbed water that may cause polymerization (and thus clogging). However, I don't quite understand how this would translate in modifying the pillar surface to suit normal phase, or other modes. As far as my knowledge goes, the silicon forms silicon dioxide spontaneously, and the surface should, therefore, be modified just as easily as silica-based packed columns.
Dear Sir. Concerning your issue about the use of pillar array columns (PAC) for separations . In this application note, plant extract samples are used to compare the analytical performance of a µPAC™ nano LC column to state-of-the-art UPLC columns. The combination of superior separation performance and increased detection sensitivity will enable analytical scientists to identify even more components in a single metabolic proling run. I think the following below links may help you in your analysis:
http://www.pharmafluidics.com/wp-content/uploads/Application-Note-Metabolic-Profiling.pdf
Thanks
Thank you for answering.
What exactly do you mean with HILIC? For that mode, you need a polar stationary phase, and the pillar array format is only available in RP mode. Pharmafluidics only sells C18 end capped coated pillars. This is exactly why I started to wonder about the other separation modes. I understand that when the interpillar distance is too small, it would clog. However, if C18 modifications are possible (which is a very long chain, longer than others), then this made me think that other coatings should be possible as well... but somewhere here in the thinking process I must be making a mistake because I have not found any paper discussing this, or other modes with pillar array columns.
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