Pierce phosphatase inhibitor mini tablet solution can you store at -20 degree C?

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What are the roles of both Monetry and Fiscal Policy coordination in optimising the Macroeconomic outcomes in the economy ?

These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...

30 July 2024 2,104 6 View

How to get recent research on poultry production?

poultry production in nigeria

29 July 2024 5,518 2 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What can I do if primer solution gives band in electrophoresis before PCR reaction?

Trouble 1: I ran a PCR reaction and found a low molecular weight band (100 bp) when doing electrophoresis. 1ng plasmid (OD260/280=1.84) was used as template. Final concentration of primers was...

13 June 2024 5,540 6 View

Please indicate whether you believe that the use of virtual reality has a positive or negative effect on people ?

Good morning, I am currently engaged in research in the field of virtual reality. I would be grateful if you could share your opinion on the subject. Your input is invaluable to me. Kind...

25 May 2024 8,567 5 View

Can bands of cell lysate be seen during SDS-PAGE?

I want to detect my target protein through WB and it worked well before. But now I could not get any signal for both my target and the GAPDH, so I ran SDS-PAGE. I expected many bands on the gel of...

23 May 2024 613 6 View

What is my problem when amplify a target gene from cDNA?

I want to copy a target gene from cDNA into a plasmid. The primers were designed according to the CDS sequence from NCBI. But when I performed PCR reactions I could not get any target bands. So...

15 April 2024 2,115 4 View

Can I incubate another secondary Ab after imaging with ECL during WB?

I found to use a wrong secondary Ab for my WB when I visualized it with ECL, can I wash with TBST for several times and incubate the right secondary Ab? Another question is why my bands are not...

16 February 2024 3,187 4 View

Qual è il ruolo dell’employer branding per una Employee Value Proposition di successo?

👋 Sto attualmente conducendo una ricerca finalizzata ad esplorare come le attività di employer branding possano permettere alle imprese di garantire una EVP (Employee Value Proposition) di...

17 January 2024 9,183 1 View

Questions about regulation of signal pathway through changing in protein expression level?

When I overexpressed a protein in a cell line, I found decrease of phospho-PLC (phospholipase C) at the protein level through WB. But when I knocked out the protein, there was no difference of...

10 January 2024 8,201 3 View

Is the use of microcrystalline cellulose MCC 102 in direct compression technology justified, given the low flowability of this excipient?

Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...

08 July 2024 2,807 0 View

Immunofluorescence of brain tissue?

I did a frozen section of mouse brain tissue with immunofluorescence staining. My partner and I did c-fos staining together. We used different primary and secondary antibodies for different...

20 June 2024 8,989 1 View

Can anybody guide me for How to solve polynomial equation for checkpoint analysis in formulation development"?

I have done formulation development of tablet for which i have considered two independent variable. Results are plotted in a statistical analysis software and polynomial equations are generated....

24 April 2024 3,951 1 View

Why does my protein of interest get pulldown during IP, but not its complex memebers?

Hello, I am trying to conduct an IP between two proteins (protein A- 97kd and protein B-37kD). For my experiment, I am using 0.5X RIPA buffer with the addition of protease and phosphatase...

08 April 2024 1,432 0 View

Is it wise to add phosphatase inhibitors to the PFA while doing transcardiac perfusion in rats for detection of phosphoproteins using IHC?

I was trying to visualize phospho-ERK and phospho-Cofilin in rat brain sections. However, the images were disappointing and after doing some literature search, I realized that this may be due to...

07 April 2024 3,938 0 View

How to avoid self Ligation even after phosphatase treatment..?

I am trying molecular cloning of vector 5.717 kb with 3.792 kb of the gene with a single digestion. However, I am giving phosphatase treatment, but I am still getting a self-ligated vector after...

02 March 2024 9,096 3 View

What is cause of qPCR curves to be sharp?

Hi I am getting these weird curves on qPCR Anyone knows why ? I tried to decease the primer and tablet concentration No change.

18 December 2023 5,105 2 View

How to make a paste in a soft tablet form which is easily chewable n spread in the mouth completly?

the tablet should be compressed n the mixture of paste shouldnt be heated much

16 October 2023 9,718 1 View

Gatric resistence time extension with protien tablet ?

do the ans

27 September 2023 7,986 0 View

The research of time release tablet DBNPA?

DBNPA(2, 2-dibromo-3-nitrilopionamide) is an ideal environment protection agent as biocide-algaecide and water treatment agent.

22 September 2023 6,365 1 View