Hello dear reasearchgate fellows,
I am interested in culturing Physcomitrium patens as a heterologous system. May i know some best ways for long term preservation of the species please ?
Hi, I have an inventory designed for USA . I want to use it for immigrants in UK. Should I have to validate it for each of subgroups like for Indians, Chinese , Thai and etc of my selected population?
28 January 2024 6,744 2 View
I am trying to change the colors of the same spectra for different areas in the same curve using top spin 4.0.7 for 2D NMR..I changed the curve color but it is applied to the whole curve.. But I...
02 January 2024 5,698 0 View
i have tried to check the CFU of 6 species of bacteria using pour plate method, and all the bacteria had some smeared colonies within the agai medium.
26 October 2023 6,726 1 View
give me brief explain
12 October 2023 9,209 0 View
If you have any guideline based on your institute, please share
13 August 2023 6,795 1 View
Please guide me. I synthesized Iron (Fe3O4) nanoparticles which have magnetic properties and got a sharp peak Uv Vis Spectrophotometer (SRP) 193nm. But in the literature, I could not find any...
06 August 2023 8,013 3 View
In the different construction context, all standards are not similar. therefore, the available BIM objects are suitable or not.....
17 July 2023 9,162 1 View
If anyone has used ABTS peroxidase in ELISA as the substrate, how long do you incubate before you stop/read the plates? I am going to develop an ELISA and plan to use ABTS peroxidase and aim to...
31 May 2023 1,465 4 View
What legal facts develop a closure to develop the significance of environmental impact aspect registration in the poultry industry? How to use the environment pollution method as a rating for...
12 March 2023 8,682 0 View
i find several articles for deposition by spray or different method, but not CBD. if any one can help me i will be so thankful..
21 December 2022 6,975 3 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
24 June 2024 2,609 3 View
I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...
15 June 2024 5,515 5 View
Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...
10 June 2024 4,152 1 View
I'm trying to culture the cell lines H69 and H69AR. Regarding the parental cell line, I'm finding my way in the handling, but with H69AR it's been troublesome. Currently, I'm culturing H69AR in...
05 June 2024 3,471 0 View
Hi Everyone! Currently, I am culturing the BM-MSCs, but it is taking very long, and the yield is very low. I am using DMEM+10% FBS+L-glutamine and I tried the Mesencult media as well. Please let...
02 June 2024 2,140 0 View
I am culturing HEK293 cells. When I plated these cells in a 96-well plate, the cells looked healthy and there were no precipitates. However, after I dosed them with the drug RITA, they developed...
30 May 2024 5,169 2 View
Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...
28 May 2024 1,398 0 View
Hi everyone, I am culturing colorectal cancer organoids (Control and Experimental) and I observe a faster growing (number and size of organoids) in experimental condition. Now the question is,...
27 May 2024 9,258 0 View
I am having a difficult time finding such a supplement, it's either discontinued by the manufacturer or for bovine or mice.
30 April 2024 6,993 0 View