Hello dear reasearchgate fellows,
I am interested in culturing Physcomitrium patens as a heterologous system. May i know some best ways for long term preservation of the species please ?
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
18 January 2021 5,438 2 View
Is extraction control also add to the qPCR mixture with the sample or does it run separately? Can anyone please explain their importance and how to use them in q PCR protocol? Thank you.!
23 November 2020 314 3 View
Difference and advantages and disadvantages of using recombinant proteins versus chimeric proteins as antigens in serological tests
28 September 2020 5,307 3 View
I am looking forward to developing an ELISA which can catch both anti-Toxoplasma IgG and IgM antibodies in Marsupials. Has anyone have any thoughts about detecting two different antibodies ( IgG...
24 July 2020 4,193 3 View
Dear Researchers: Greetings! I am interested in learning electron microscopy image analysis of bacterial biofilms, mainly using open-source software. I have been using basic functions and tool...
28 May 2020 5,572 4 View
When the condition has length > 1 in ifelse in r and there are more than 2 statements how to use ifelse? I have a data set wherre I want to categorise people in to categories using sveveral...
04 March 2020 4,811 6 View
I have started a study of record available at clinic collected years back.So it seems to be a retrospective cohort study.But when calculating sample size I feel like it to be done in normal cross...
12 August 2019 866 3 View
08 May 2019 7,631 2 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...
22 February 2021 8,253 1 View
Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...
04 February 2021 10,018 5 View
Hello there! My lab has recently been (very graciously) gifted an array of cell lines, of which include 67NR and 4T07 cell lines. While I've found a decent amount of background and culturing...
02 February 2021 2,590 3 View
Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...
02 February 2021 7,892 4 View
Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...
01 February 2021 7,153 4 View
Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...
24 January 2021 7,223 3 View
I have faced a problem with culturing Ac16 cardiomyocytes cell line. Day 0 I plated 200,000 cells/2ml (well) in 6 wells plate in FGM. Day 3 I put my cells on Low serum medium. When I changed...
24 January 2021 1,456 2 View