09 September 2019 6 5K Report

The size of protein X (Phosphorylated and non-phosphorylated) will be pretty much the same on a western. Let's say after primary antibodies incubation of the two separate proteins (Phosphorylated and non-phosphorylated) on the membrane; can you pick specific secondary antibodies (one for Phosphorylated and one for non-phosphorylated) that you can in theory detect both bands (Phosphorylated and non-phosphorylated) on the same membrane.

Probably using secondaries antibodies detected with IR fluorescence.

Anyone ever tried this approach?

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