Vivica Grotelueschen

5 Questions 131 Answers 0 Followers

Questions related from Vivica Grotelueschen

Does anybody know a supplier for tmTCEP (trimethyl TCEP)?

I found different publications about methylated TCEP which state that tmTCEP has a lower pKa value (4.5) and therefore works faster at low pH values, but there was no information where the authors...

03 March 2017 2,664 3 View

Which protease inhibitors are acetone soluble?

I know that protease inhibitors are typically dissolved in water, alcohols or DMSO, so the suppliers normally don't test for acetone solubility. Anyway I'm looking for protease inhibitors (serin...

01 January 2016 6,737 3 View

Is it possible to separate two antibodies bound to each other without interrupting binding to protein G resin?

I'd like to bind antibodies of species 1 to a protein A (or protein G) resin and to catch antibodies of species 2 that are cross reactive against species 1. At the end I have a sandwich of Resin...

06 June 2014 3,286 3 View

Advantages and disadvantages of sodium azide/thymol/thimerosal?

Typically most labs use sodium azide as a preservative, but there is also thymol and thimerosal that are also well-known preservatives. Is there a reason, why azide is used much more often than...

04 April 2014 5,002 11 View

What is best way to prepare a human erythrocyte ghost, and what is the best way to store them?

I need the blood group antigens of the erythrocyte membrane for my research and want to prepare ghosts out of EDTA blood. At the moment I just wash the erythrocytes in 0,9% NaCl, lyse in distilled...

01 January 2014 5,203 3 View