I prepared a Phosphate Solubilizing Media in
200ml distilled water with
Glucose(2gm/200ml),
Tricalcium Phosphate (0.25gm/200ml),
Magnesium Chloride(1gm/200ml),
Magnesium Sulphate(0.05gm/200ml),
KCl(0.04gm/200ml),
Ammonium Sulphate(0.1g/200ml),
Agar(2%).
The flask was kept in a shaker with a magnetic stirrer while adding the above components to make sure all the components dissolved properly. After it was dissolved properly, pH was adjusted to 7 and autoclaved. After autoclaving, it was found that the bottom of the flask had clotted media unfit for plating. Is this case normal? How can we minimize this? I repeated the above process twice and got the same results.
I suspect that it could be some kind of floculation promoted by magnesium sulphate. Have you considered using Pikovskayas Agar instead?
https://us.vwr.com/assetsvc/asset/en_US/id/8042423/contents/himedia-pikovskayas-agar-m520
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