Can anybody provide me a scientific reference on how to prepare PBS solution? Thanks!
Millonig, G. (1964). Study on the factors which influence preservation of fine structure. In “From Molecule to Cell" (P. Buffa, ed.), p. 347.
My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...
17 June 2024 4,183 3 View
1.1.- Reducir los obstáculos de las personas víctimas o posibles víctimas de trata con fines de explotación sexual y sus hijos/as en su acceso a recursos, prestaciones y ayudas forales. 1.2.-...
10 March 2024 8,166 1 View
I'm planning a project focused on studying osteocytes in the long bones of rats, requiring intact sections of both cortical and trabecular bone. To achieve this, I'm considering Kawamoto's tape...
23 January 2024 3,172 2 View
Error [65]: Error, extent of vector too large or attribute table error." I uninstalled and reinstalled QGIS 3.26.2 but I still get the error. Any idea what is causing this?
07 August 2023 4,797 1 View
Hi, I would like to know if someone knows the composition of the Binding Buffer from the Dead Cell Removal Kit from Miltenyi Biotec. Have you tried a home-made alternative?
15 November 2021 4,803 1 View
In photdegradation experiments using P25 or TiO2, its important to know carrier life time, because high carrier life times are desirable for avoiding recombination and benefits redox reactions of...
13 October 2021 5,046 3 View
Hi everyone! Im performing some supernatant IPs after serum starvation treatment to analyze whether my protein of interest is secreted or not. Since some cells might die during procedure, I can’t...
24 June 2021 9,299 2 View
Hi everyone, Hydroxy naphthol blue reagent is marketed in two variants, the original (CAS 63451-35-4) and in the form of sodium salt (CAS 165660-27-5). For its use in LAMP-PCR, the Hydroxy...
16 May 2021 6,960 4 View
I am looking for studies on the phenomena of urban-rural exodus due to the Covid-19 pandemic in Latin America and southern regions of the planet. Can anyone suggest studies?
16 April 2021 4,998 2 View
Hi all, We filtered seawater and preserved the filters in RNAlater on site. The tubes with the sample in the RNAlater were placed in a cold box until arrival in the lab where they were left...
07 February 2021 4,022 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View