How can I make interpretation of kendall's Tau-b correlation magnitude?

Hi. do somebody know if Kendall's tau-B value of 0,06 or 0,11, 0,14, 0,20 is a fair or weak association? or how to make interpretation? Thing is, we are writing a descriptive study, the sample...

14 January 2020 6,514 7 View

Where do I order amplicons/plasmids from specific primers to make standard curves for QPCR?

I trying to quantify total fungal populations in water and biofilm using QPCR. I read that you make the standard curves using plasmids that contain the specific amplicon. I was wondering if there...

21 January 2019 980 3 View

Is there an effect on the isotopic signatures of zooplankton, if there is salt on some of the dried samples?

16 January 2019 3,850 1 View

Is there any report of fungi or endophytic bacteria causing diseases in algae or macroalgae meadows or marine ecology?

How can one identify the affectations in algae produced by parasites? How can we identify the effects on algae caused by fungi?

15 February 2018 9,871 2 View

How to optimize solubility of my heterologous protein overexpressed at 0.04mM of IPTG and 26C incubation ?

Good morning/afternoon I am over-expressing a bacterial protein (dimer) in BL21 E. coli, which it works even with 0.04mM of IPTG, incubated at 26C. However, when I want to check the solubility of...

20 August 2017 2,306 5 View

What are the best transgenic wistar rat models for Alzheimer's disease?

There are different transgenic rat models with on-off switching genes but I would like to know which of them have early alterations in behavioural tasks.

14 March 2017 195 2 View

Could burned land be recovered?

The bioremediation is used for recovery contaminated lands, but is possible recover a land burned?Bacterias that use carbonized matter? Maybe is a crazy idea...

06 February 2017 9,574 5 View

Which reference electrode is better for SPE, Ag or Ag/AgCl?

12 April 2016 5,382 10 View

Does anyone know of research about Latin American new media? Is there any list of new media in Spanish?

I'm studying the new models of digital journalism and entrepreneurship in Spanish. I did it for the Spain case, and I want to improve it to all the Spanish speaking countries. Do you know people...

14 May 2014 1,061 3 View

Can anyone recommend some publications withe the results of elisa asssay?

Seeking help with Elisa assay

30 June 2013 970 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View