I'm planning a project focused on studying osteocytes in the long bones of rats, requiring intact sections of both cortical and trabecular bone. To achieve this, I'm considering Kawamoto's tape sectioning technique for frozen blocks. My concern is about effectively adhering the tape, with the tissue section facing upwards, to ensure it remains attached to the glass slide throughout the entire FISH protocol. I'm interested in knowing if anyone has successfully used this technique for FISH applications with any type of tissue.