04 January 2023 1 2K Report

Hello, now I am processing a 300kv cryoEM data by relion for a small membrane protein(~100kd), which only has transmembrane domain. However, when undergoing 3d classification, I found protein model didn't stack well. It seemed multiply slides in the model. Also the model looks not so compact but a little scattered. Does anyone meet this problem before, or has some suggestion during data process?

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