Is a wall with constant pressure and constant inlet flow similar to an inlet boundary?

At an opening in turbulent flow, using wall boundary condition, with constant flow rate and constant pressure inlet, would be similar to an opening with inflow BC?

03 February 2021 2,390 5 View

Is it possible to estimate the most recent common ancester using o & 1 data? If yes would you please introduce a software?

Dear professors Respectfully, i know that sequences can help us to reveal the most common ancestor in phylogenetic studies. However, in genetic diversity studies using IRAP, SSR, markers etc, i...

13 November 2019 9,305 6 View

Identify DNA bands transfer from gel to the membrane(southern blotting)?

Hello Is there a way to detect transferring of DNA bands from gel to membrane in southern blotting without staining? Thanks

10 November 2017 7,804 3 View

Can anyone suggest the best method for measuring PON1 arylesterase activity in serum?

05 January 2015 3,060 1 View

Is there a possibility to see any green substance in extracted DNA?

I saw a green substances in potamogeton DNA. Does anybody know anything about this?

10 May 2014 5,702 4 View

What is the cause of green DNA in Potamogeton extracted DNA and how can I delete that?

Green DNA of Potamogeton and deleting the color.

05 May 2014 9,649 3 View

Hydrological test statistics

What is the best test statistic for gamma distribution?

01 January 1970 7,770 3 View

What is the best RMSE value for rainfall data?

rmse and mad

01 January 1970 8,622 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View