Hello everyone
Is there any phage that use retrotransposition?
I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...
01 June 2024 338 2 View
Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...
26 May 2024 2,151 6 View
I faced with a problem, and I think you can help me regarding this issue. I have sent one of my PCR poducts (ITS1-ITS4) to a company in order to sequence it. Indeed, I sent them various PCR...
01 February 2024 2,626 3 View
What is your solution regarding extra bands in ITS molecular markers? I am trying to use ITS1 and ITS4 markers in 15 plant species. Unfortunately, I only received extra bands instead of one after...
28 November 2023 9,305 4 View
I've recently faced a question that I couldn't solve on my own or with colleagues. I want to know what are PCR biases and how to handle them? Any answer in welcome.
20 June 2023 9,275 1 View
Hi all, I have two questions regarding taxonomy 1- how do you define a novel family? or even order? 2- if you have a strain that is not deposited in two culture collection, can you describe it...
20 June 2023 6,362 1 View
I have two morphologically similar species that differs molecularly only by 4 bases. Can I synonymmized them? If not, how many base differences can lead to this taxonomic revision? How do I deal...
10 April 2023 3,602 18 View
I want to isolate only yeasts from a sample and I want to know if there is (a) compound/compounds that I can use to inhibit the growth of gram-negative bacteria in my culture media. If that...
06 October 2021 2,978 6 View
Has anybody had any issues with Rotenone precipitating out in cell culture medium. I see brown precipitates forming in cell culture media after 1-2days in culture at doses above 30uM. If so, what...
28 July 2021 4,002 0 View
I extracted DNA from some kombucha products with a manual protocol, with one final step using phenol, for 16S and ITS sequencing. However, there is a problem for the majority of my samples in PCR...
12 June 2021 9,655 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View