Any thoughts on what is the preferred method for glucose feed control in E.coli fed-batch? Both seem to have their merits, but quite difficult to tune pump PID settings to avoid sub-optimally controlled feeds with either approach. Also, for pH-stat, response is dependent on media buffering capacity.
pH –stat
pH -stat titration is applicable to any bioreaction involving pH variations. Up to now, the main application of the pH-stat titration has been for nitrification monitoring. In this article, we present a theoretical model of pH-stat titration, which predicts the response to any reaction involving the production or consumption of protons, hydroxyl ions, or inorganic carbon chemical species (CO(2), HCO(3)(-), CO(3)(=)). This model is a useful tool to understand pH-stat titrations, to define their applicability and limits, and to select the best experimental conditions for specific applications. Tests have been performed to compare experimental pH-stat titration rates in the presence of carbon dioxide and HCO(3)(-) producing reactions to the values predicted by the model and a very satisfying correspondence was found.
A pH-Stat study takes place as follows:
1. determining an optimum value for the pH of the studied reaction,
2. keeping the pH constant by adding a reagent to neutralise OH or H3O+ ions,
3. calculating the kinetics of the studied reaction based on the consumption of reagent required to keep the pH constant over time.
Entedhar Sarha : Please stop plagiarizing other people's published work and presenting it as your own on this and other websites.
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