7 Questions 3 Answers 0 Followers
Questions related from Christopher P Marquis
I am wanting to sparge tube cultures of a bacterial culture to promote gas transfer and provide some mixing in tube cultures. Can anyone recommend an optimal choice between: A (145-174 micron), B...
21 January 2021 8,253 0 View
Does anyone know if sodium azide is cytotoxic to mammalian cells (293, CHO, murine hybridomas) at 0.1-0.2% w/v? Buffers such as MACS buffer seem to include it but I would have thought this would...
12 April 2018 3,538 3 View
Any thoughts on what is the preferred method for glucose feed control in E.coli fed-batch? Both seem to have their merits, but quite difficult to tune pump PID settings to avoid sub-optimally...
12 January 2018 310 2 View
Can anyone point me to some useful references on methods to assist in clarification of crude homogenates of animal tissue? Centrifugation in our case is only partly successful and our current...
29 April 2014 2,826 1 View
We have purified some of Fabs and would like to get some feedback on the best way to store them. Ideas on • Which buffer? • With/without glycerol? • 4oC or -20 oC? Would be greatly appreciated.
11 December 2013 1,384 2 View
Any suggestions of culture collections or other sources where we could obtain this culture?
15 October 2013 6,556 2 View
Secreted recombinant transient protein expression
20 February 2013 3,417 13 View
