No you can't I think. But you can make a sticky end to your blunt end PCR product. There is a cheap and simple trick to it. Just add Taq Polymerase (up to 1.0 units) to your blunt-ended PCR product and incubate further at 72 C for at least 10-15 minutes on the PCR machine. Then place your reaction mixture onto ice and use it immediately for ligation. I have done this many times and it works pretty well for me.

Well kind of. The way you would blunt end it would be to add an enzyme to chew back the overhang. You can do this using T4 DNA Polymerase, but it is in my opinion easier to go the route I recommended earlier.

Hi Zach,

Possible to chew the plasmid?