How do I normalize on three reference genes with Pfaffl Method in RT qPCR?

Hi, I have a question on RT qPCR: until now I used Livak method to analyze my qPCR data. I normally use 8 different reference genes and determine the three most stable ones. Then I use the...

10 October 2018 3,719 2 View

How to get rid of persistent cell culture contamination?

We are having a problem with contamination in our cell culture. Since a couple of months we are getting frequent but isolated contaminations with bacteria (we think it might be E. coli). We...

21 January 2018 2,650 3 View

Any advice on how to derive clones from hIPSCs, stably transfected with inducible overexpression constructs (problem: spontaneous differentiation)?

I need to derive human IPSC clones from a bulk population that was stably transfected with Dox-inducible (TetOn) overexpression constructs, using the piggybac / transposase system. I did the...

31 October 2017 1,312 2 View

What process variant of shear cutting is applicable for shearing of fibre reinforced plastics?

For some application is it necessary to achieve a high qualitiy of the cutting surface. To what extend it will be possible to transfer the effects from shearing of sheet metal materials to...

14 May 2017 2,847 3 View

Coral/Mangrove Conservation Work in India?

Looking for marine scientific contacts all around India, especially people working in Coral and Mangrove Conservation? Who knows where institutes are located, where projects can be joined and...

22 November 2016 9,173 6 View

Does anyone know how storing fresh soil samples in a vacuum sealed bag affects soil microbial activity?

I need to store fresh soil samples for several weeks and want to avoid lysing microbial cells (so I cannot sterilize the soil), but at the same time minimise microbial activity.

08 May 2016 194 6 View

How can one measure sex hormones in mice?

22 October 2015 4,236 5 View

Is there an immortalized olfactory ensheathing cell line?

Dear all, is there an immortalized olfactory ensheating cell line avaiable on the marked? I would like to purchase OEC rather than preparing them freshly.

14 October 2015 1,916 3 View

What are the ethical implications of cognitive robots?

As we strive to create embodied artificial intelligence with increasingly greater capabilities, do we have a moral responsibility to set ourselves an acceptable limit to the level of intelligence...

13 September 2012 3,127 16 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

When I mix oil and NaOH solution, Why solid layer in the surface is formed?

During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...

22 February 2021 2,116 4 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...

19 February 2021 2,787 1 View

Which compound is getting oxidized during bulk electrolysis with TFAA?

The experiment is really simple. When I do a bulk electrolysis with 1% TFAA (ACN solution) to get dihydrogen, what is going to be oxidized? (working electrode graphit, counter electrode platinum,...

18 February 2021 4,430 1 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View