Peroxide value of seed oil: Sometimes the consumption of titration solution of the blank value is higher than of the oil sample. Why?

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Why does an agarose gel stained with EtBr stay partly dark?

I ran a gel electrophoresis with a 1.5% agarose gel stained with 0.5 µg Ethidium Bromide per mL of agarose. When looking at the gel under fluorescence, one half of it remained dark and did not...

09 April 2024 5,033 3 View

Relative permittivity extraction - Python or Matlab algorithm taking into account the Free Space Measurement Method?

Hello together, Transmission and Reflection Free-Space Method have been used to get S-parameters of our MUT. As a next step we used scikit-rf for time-domain analysis and gating. Is there a free...

10 December 2023 2,771 2 View

How to test whether isolated human neutrophils are functional or not?

I am trying to understand whether my isolated neutrophils are functional or not after isolation. Therefore, I would like to compare the functionality of freshly and cryopreserved neutrophils,...

22 October 2023 5,709 6 View

Does anyone know a good method for measuring the effect of a substance on Pseudomonas spp. occurrence in soil?

Normally I would extract the DNA and then sequence it, but this is just a small experiment, which need do be done fast. I looked up selective media for Pseudomonas spp. and found a few: CFC agar,...

18 September 2023 3,381 3 View

Cut and paste cloning results in no colonies, why?

Hi, I try to clone a V5-TurboID (1Kb) into a roughly 6kb big backbone (pEGFPC2). The restriction enzymes used are NheI and XhoI. Both fragments are gel purified and ligated with T4 Ligase (1h or...

30 May 2023 6,249 4 View

How to sequence PCR products created with degenerate primers?

Hey everyone, I used degenerate primers to create PCR products. They showed a single strong band on the gel. First I tried to send them in with the degenerate primers, but -as expected- it was not...

16 March 2022 3,006 2 View

How to calculate the IC50 in Prism?

Hello, I am using Prism to calculate the IC50 of different chemotherapeutics. This time, my concentrations are between 0.25 and 1.5 microgram/mL (0.25, 0.5, 0.75, 1, 1.25, 1.5). Converting the...

03 November 2020 6,752 3 View

How to dissect a Lepidoptera larvae (Galleria Mellonella)?

I want to isolate the microbiom from the gut of the larvae but I have problems with the dissection. I tried to use needles and a scalpel from a dissection set for biologists but I had problems to...

27 July 2020 3,994 6 View

CRISPRi and CRISPRa - transfection or transduction?

Hi everyone, we recently started using CRISPRi and CRISPRa in our lab and we are struggling to get good results - mainly with CRISRPi. For the gRNAs we used 5 different gRNAs alone or in...

28 June 2020 4,715 3 View

How do I normalize on three reference genes with Pfaffl Method in RT qPCR?

Hi, I have a question on RT qPCR: until now I used Livak method to analyze my qPCR data. I normally use 8 different reference genes and determine the three most stable ones. Then I use the...

10 October 2018 3,850 2 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

Do you think can be any gas and oil bearing rocks in Eastern part of Iran?

I want to know more about petroleum deposits in Iran.

02 August 2024 8,725 3 View

Heavy Crude oil PVT Data?

Hello i need heavy oil PVT data having viscoisty of 2000-3000 cP. If someone have it or research papers about it, kindly share it with me Thanks

25 July 2024 6,940 1 View

What are the most PROBLEMS of HYBRID nanofluid in oil industry ?

explain your idea

23 July 2024 1,197 0 View

Do you think can be any gas and oil reservoirs in central of Iran?

I want to know more about gas and oil reservoirs in Iran.

14 July 2024 3,350 2 View

How am I supposed to find justifications for the unusual fatty acid profile in broiler diet?

I've obtained a fatty acid profile of broiler diet comprised of SFA 48 to 93%, MUFA (22 to 40%), PUFA (non determined to 10.4%. The PUFA composition is mainly n-6 and almost no value for n-3. The...

08 July 2024 9,165 4 View

Determination of fatty acids in fish?

I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...

02 July 2024 900 1 View

Is there a vegetation zone dividing location based on climate or geographic parameters available by province level?

I am researching the traceability of palm oil and need a parameter that can differentiate between provinces around the world. I want to group the provinces by their vegetation zone and see if it's...

01 July 2024 888 2 View

How to know if the oil palm tree is functioning properly in terms of absorption of different nutrient?

The idea is to design an IoT system that is able to capture real-time conditions from an oil palm tree to ensure optimal growth. Nutrients such as Sulfur, Nitrogen, Boron, Zinc, Copper and Iron.

30 June 2024 3,046 2 View

Effectiveness and Implementation of CCUS in Oil Fields: Genuine Progress or Demonstrative Effort?

Many studies have been conducted on Carbon Capture, Utilization, and Storage (CCUS) in oil fields to increase the recovery factor. Do you believe these studies are genuinely useful, or are they...

24 June 2024 7,441 0 View