Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

How can we explain the decrease in taste in a patient with desminopathy (myofibrillar myopathy)?

A patient with hereditary desminopathy (mutation Thr341Pro DES in a heterozygous state) with disease progression has a significant decrease in taste. How can this fact be explained?

15 September 2020 3,742 4 View

Can Protein A tag interact directly with secondary antibodies on western blotting?

Hi, everyone! Does anybody have experience with WB of ProtA tagged proteins? Can it give a non-specific signal when you probe the membrane with control ab to different size protein?

29 November 2017 5,953 1 View

Can antibidy react with loading buffer?

Hi, I'm trying to perform some blotting with yeast Trf4 protein, but each time get strange result with nonspecific spots. It appear even in control wells, filled with water+loading dye, but not in...

26 June 2017 3,477 2 View

No title

05 June 2016 8,332 8 View

Is anyone else having problems with freezing tissue samples prior to cryostat sectioning?

In our lab, we freeze tissue samples from mice (embedded in OCT); we used to have a few problems with this method, using the standard plastic 15 mm cryomolds (frozen with dry ice & alocohol;...

01 January 1970 502 4 View

Is it necessary to reduce the level of uric acid in the blood of a patient with desminopathy (myofibrillary myopathy)?

01 January 1970 422 3 View

How can I reduce the level of the pro-inflammatory cytokine interleukin-6 (Il-6) in myofibrillar myopathy?

Over the past six months, a patient with hereditary desminopathy (Thr341Pro DES mutation in the heterozygous state) has a 1.6-fold increase in the level of the pro-inflammatory cytokine...

01 January 1970 3,273 6 View

Frozen sectioning as a method for research

What could we change in preparing tissue samples prior cryosections(cryostat) method for more convenience and results? ( I mean cryo-embedding) The second question is in which areas of research...

01 January 1970 679 3 View

How can I reduce the level of C4 complement components for desminopathy?

Within three years, a patient with a desminopathy (Thr341Pro DES mutation) was found to have a 17% increase in the level of C4 complement components to 0.41 g / l (Norm 0.1-0.4 g / l).

01 January 1970 9,949 2 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Receiving very less number of hits and events in acoustic emission tests?

I am doing a three point bend test with concrete specimen of dimension 280mm*90mm*90mm. I am putting 2 sensors near the notch with 3cms away from both the sides. the experiment ran for 20 mins but...

13 February 2021 2,990 1 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

Acoustic simulation of Membrane?

I have 1 mm thick membrane. This membrane is perforated. I want to simulate this in Acoustic module of comsol. For this ,which type of module I can use in comsol.?

02 February 2021 2,505 6 View