PDT induce "naked nuclei" (cytoplasmic membrane lysis with hole nuclei). What is the fate of "naked nuclei"? Apoptosis or necrosos? - FAQS.TIPS

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Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

What is going wrong with my IP solution for the patch-clamp?

Hello. I moved to a new lab. My IP solution, which I used at previous labs, stopped working. I can not hold a cell for more than 5-7 minutes, then leak current increases dramatically and I lose...

29 May 2024 2,291 4 View

Why are the following muscles affected at the onset of desminopathy: Semitendinosus, Gracilis and Sartorius?

It is known that in the early stages of desminopathy the muscles most often affected are: Semitendinosus, Gracilis and Sartorius. What is the reason for the damage to these particular muscles?

13 January 2024 9,691 7 View

R.M. Smith, D.J. McMahon, E.J. Griffith, Conduction drying of meat: a review, Journal of Food Engineering, vol. 92, no. 3, pp. 473-482, 2005?

Conduction drying of meat

18 July 2023 385 3 View

How to explain the similarity of botulism and desminopathy (myofibrillar myopathy)?

In a patient with desminopathy (mutation Thr341Pro DES in the heterozygous state) with the progression of the disease, we note signs and symptoms that are also characteristic of botulism:...

18 June 2023 2,525 6 View

What accumulations of inclusions do we observe in a muscle biopsy of a patient with desminopathy?

When staining with hematoxylin and eosin of a muscle biopsy from a patient with T341P desminopathy, we observe accumulations of inclusions similar to nuclei (arrows in figures 1 and 2, x280). And...

09 February 2023 3,220 4 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 6,107 6 View

How can we explain the decrease in taste in a patient with desminopathy (myofibrillar myopathy)?

A patient with hereditary desminopathy (mutation Thr341Pro DES in a heterozygous state) with disease progression has a significant decrease in taste. How can this fact be explained?

15 September 2020 3,922 4 View

Can an increase in blood residual nitrogen and urea worsen the condition with desminopathy (myofibrillar myopathy)?

In a patient with hereditary desminopathy (Thr341Pro DES mutation in a heterozygous state) with disease progression over the past 7 years, the level of residual nitrogen in the blood increased 1.5...

07 April 2020 8,142 0 View

Safe and reliable method to seal a spinal cord to agar?

Hello! I need to prepare a thin slices from mouse's spinal cord. The problem is, that I can't use cyanacrylic-based glue due to it's cytotoxicity. If I put spinal cord to agar cube without a...

24 September 2019 504 0 View

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Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

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Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

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How to represent Annexin V apoptosis study data in publications?

Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...

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