PD-10 Desalting Column: how much DMSO it can tolerate?

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Signal phase shift after analytical calculation?

Suppose we have a signal as x(t) = exp(i(omega*t + phi)), after a simple calculation as y(t) = 1/x(t). We can clearly see that there is a phase shift of Pi=180deg. However, using directly...

20 October 2020 4,923 5 View

Any recommendations on the software to do text mining or sentiment analysis on Chinese reviews?

I am trying to do text mining on Chinese reviews. I have tried out many softwares, like the RapidMiner, Chinese Text Analytics, Python. Most of them seem to require certain level of programming...

12 August 2020 9,109 8 View

HEK-293t cell detach but still alive during killing curve set up?

Hi there, I'm trying to build a killing curve for HEK-293T towards hygromycin B for the following transfection experiment. I plated the cell at 1.3X10^5 cell/well in the 24 well plates the night...

30 June 2020 7,368 1 View

Is my enzyme not working?

Hello everyone, I am currently working on a cloning project with 2 vector pBin mCherry (for localization) and pG5H-GFP (for gene expression). I need to clone 1 ATG gene with the size of 1.3 kb...

09 February 2020 7,931 2 View

We transfected HEK cells for a secreted protein and want to confirm the protein is expressed in right MW. Should we collect supernatant for Western?

We transfected HEK cells for a secreted protein and want to confirm the protein is expressed in right MW. Should we collect supernatant for Western? Not sure we choose the right method. We have...

14 May 2019 4,804 4 View

Why should SVD get two equal singular values for a single mode?

Singular Value Decomposition (SVD) is an important method in the area of signal processing. I try to use SVD to calculate the MHD mode structure from experiment data. However, during the benchmark...

10 April 2019 6,118 4 View

How to derive the Boltzmann equation in plasma physics?

Boltzmann equation in plasma physics is a very fundamental equation in kinetic theory. What's the relation of the one in plasma physics and that in thermodynamics.

03 April 2019 4,925 8 View

What the best soltion has good adhesion to CdS thin film?

I'm trying to find a solution to dissolve particles CZTS- about 15nm.This solution has to have great adhesion to CdS layer. Recently, I have tried toluen and hexanthiol but I still can control...

15 March 2019 5,444 2 View

How to clone a gene with long sequence?

Dear All, I'm now trying to amplify a gene with 3690bp from the genomic DNA. I've design 3 sets of primers. First set is flanking the whole gene. Second set is flanking from 1 to 2613bp. Final...

07 February 2019 1,167 1 View

What bending test and torsion test standard are recommend for a surfboard?

I wish to find a bending and torsion test standard for a surfboard (non-prismatic) that is roughly 2000mm long. The closest I've found for bending test is probably those ones that are designed for...

04 February 2019 3,222 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View