i have extracted bacterial genomic DNA, and after running multiplex PCR on it, i got blurry thick bands below marker. the marker used is 100 bp. i checked using nanodrop and diluted the DNA conc to 50pg. any advice would be useful.
These are primer dimers and unused primers. You may consider using lower amounts of primer.
SD
These are primer dimers and unused primers. You may consider using lower amounts of primer.
SD
I agree with Sibnarayan and additionally if the band persists and you really want to get rid of it use a hot start enzyme as well as less primer
I recommended both Sibnarayan and Paul answers...
Also, negative control can be really helpful.
Best luck
Add a negative control (reaction tube without DNA sample) to see whether this 2 bands still present. If so, these 2 bands are most likely not amplified from your DNA template. It always good to add a negative control in your PCR experiment.
I agree with all the answers but I suggest to try using less amount of DNA extract than the used amount before
Good Luck
I think it is primer dimers you have to revise the primers volume and concentration.
Good luck
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