Hi, I am previously administered natural substances to mice pups. The literature says it was available since postnatal day 1 but starting from postnatal day 4-5 is a good approach using size 10 polyethylene tubing.

The critical point is the oesophagal tissue was very thin and easy to rupture.

I attached an article that you can refer for this method :

Article Administration of Substances to Laboratory Animals: Routes o...

Hello Tomson Kosasih

I agree with Ahmad Kurniawan . You could also refer to the two articles attached for the protocol.

Article Probiotic Studies in Neonatal Mice Using Gavage

Article A novel method for oral delivery of drug compounds to the ne...

Best.

Thanks Ahmad Kurniawan and Malcolm Nobre for sharing. That makes sense, waiting until PD 4/5 somehow might be a good time to start gavaging since their tissues have matured enough to receive gavage handling.

Your inputs are very well appreciated.