Hi, I am previously administered natural substances to mice pups. The literature says it was available since postnatal day 1 but starting from postnatal day 4-5 is a good approach using size 10 polyethylene tubing.
The critical point is the oesophagal tissue was very thin and easy to rupture.
I attached an article that you can refer for this method :
Article Administration of Substances to Laboratory Animals: Routes o...
Thanks Ahmad Kurniawan and Malcolm Nobre for sharing. That makes sense, waiting until PD 4/5 somehow might be a good time to start gavaging since their tissues have matured enough to receive gavage handling.