Mahmoud Mohy Elhaig

22 Questions 61 Answers 0 Followers

Questions related from Mahmoud Mohy Elhaig

Is it possible to use a previous prevalence rate of tuberculosis in a neighboring country to calculate the sample size for my study?

Is it possible to use a previous prevalence rate of tuberculosis in a neighboring country to calculate the sample size for my study in my country, knowing that there is no information in my...

19 June 2025 2,424 5 View

Can I convert reverse nucleotide sequence to amino acids and GenBank submission?

The AB1 sequence file sent by Macrogen company was reverse sequence only. I can use it instead of forward sequence to deposit in the gene bank and convert it to amino acids and perform analyses on...

20 August 2024 5,370 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

12 August 2024 6,818 1 View

Is it possible to calculate sensitivity, specificity, and accuracy between two serological tests without a gold standard test?

Is it possible to calculate sensitivity, specificity, and accuracy between two serological tests without a gold standard test during the disease prevalence test among a group of patients? If it...

04 November 2022 603 2 View

What is meant with "Unverified sequence" in GenBank data base?

During search in GenBank database I found in some sequences the following comment: GenBank staff is unable to verify sequence and/or annotation provided by the submitter. so, what are the reasons...

29 December 2017 3,278 6 View

What is the meaning of Odds ratio and Risk ratio and how can use them in epidemiological studies?

I want to know the meaning of Odds ratio and Risk ratio and how can I use them in a case study or epidemiological studies of infectious diseases and how can I interpret its results?

26 August 2016 3,384 14 View

Does anyone have experience with LAMP protocol instead of PCR assay?

I mean what are the principles of LAMP and How can I prepare my Lab for it ?

17 April 2016 6,182 2 View

How can I detect Diminazene Aceturate-resistant Trypanosoma evansi by PCR?

Here in Egypt, Diminazene Aceturate (DA) is misused by vets and farmers. It was noticed that several cases of disease were not cured by DA, after which emergency slaughter took place. I suspect...

17 October 2014 8,840 1 View

Can I deposit the two different sequences of PPR virus regarding F and N genes in Genbank under the same accession number?

I have two sequences of PPR virus of two genes (F and N genes) (complete Cds).

10 October 2014 3,187 4 View

Why did gaps appear after sequence alignment of my sheep pox virus isolates with others published in Genbank?

There are nucleotides missing or deletied in all viruses including our isolates and those retrieved from Genbank.

12 September 2014 6,954 6 View

Can you help me with a Kappa test to analyze our data?

I have a data regarding bovine mastitis, and the reviewer suspended the acceptance on Kappa test. thereby, I need your assistance urgently

08 September 2014 2,902 2 View

What is the difference between a partial and complete genome in studying the genetic diversity among microbial agents?

To study the genetic differences in a certain pathogen, from best using a partial or complete genome sequences.

06 April 2014 6,370 0 View

How can I amplify a complete genome of neethling virus of lumpy skin disease?

To study the genetic diversity of the neethling poxvirus that cause lumpy skin disease

03 April 2014 7,236 0 View

How can I study the resistance to anthelmintic drugs with babesia species?

I have DNA from babesia bovis and bigemina from clinical and non clinical cases.

22 February 2014 6,478 1 View

has any resistant babesia regarding bovine appeared and registered recently?

I need to study if there are species of babesia showing resistance to chemotherapy.

23 January 2014 6,633 0 View

What is the ideal statistical analysis test to describe the association between results of PCR and bacterial culture in raw milk?

I have been examining milk from dairy cows and buffaloes for sub clinical mastitis. The obtained results were positive for PCR and bacterial culture. I want to know if there is association between...

19 January 2014 5,206 9 View

What are the principles of LAMP method and its applications and how to calculate the concentrations of used primers?

Seeking an answer for Infectious diseases.

30 October 2013 1,171 1 View

Is there a diminazene resistant babesia strains in animals?

If so, how can I use PCR assay in detection of these strains in clinical samples?

30 October 2013 7,851 2 View

How can I study genetic diversity in babesia species?

Babesia bovis and other piroplasmes

30 August 2013 8,600 0 View

Can someone tell me how we to calculate true positive and true negative?

Diagnosis of infectious diseases

09 July 2013 5,529 7 View

Can someone advise on how to design an application of Bayesian latent class analysis on obtained data related to animal examination for a specific disease?

Trypanosoma evansi infection in camels

03 July 2013 9,801 1 View

Problem with detection of Mycobacterium by PCR in tissues.

What is the best technique for DNA extractions from tissue?

01 May 2013 3,890 2 View