PBS instead of ddH2O in sodium citrate buffer. What will happen?

19 June 2019 1 8K Report

I used PBS as my diluent instead of ddH2O when making sodium citrate buffer. Can anyone tell me what issues might occur during IHC epitope retrieval?

-Thanks

Oscar Otero Alfaro

Hi Colton

I think that it changes all. Ionic strength, pH, salt composition.... Therefore, your epitope maybe take a different conformation and would not be recognized by an specific Ab.

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