Help with IF slide staining, patchy DAPI stain and GFP quenching?

Been struggling with a slide mounted IF protocol. Here are my parameters: 1) Fixed in 4% PFA for 2 hours at 4 degrees C 2) 10um cryosections air dried on slides O/N 3) Coplin jar water bath to...

31 August 2018 7,603 5 View