Colton Robbins

2 Questions 1 Answers 0 Followers

Questions related from Colton Robbins

PBS instead of ddH2O in sodium citrate buffer. What will happen?

I used PBS as my diluent instead of ddH2O when making sodium citrate buffer. Can anyone tell me what issues might occur during IHC epitope retrieval? -Thanks

19 June 2019 8,382 1 View

Help with IF slide staining, patchy DAPI stain and GFP quenching?

Been struggling with a slide mounted IF protocol. Here are my parameters: 1) Fixed in 4% PFA for 2 hours at 4 degrees C 2) 10um cryosections air dried on slides O/N 3) Coplin jar water bath to...

31 August 2018 7,617 5 View