I am interested in FACS sorting for pancreatic mesenchymal cells at E10-E13. Could anyone recommend good cell surface markers? Many thanks!
I am not entirely sure if there are standard practices for tumour immunohistochemical preparation seeing as tumours aren't usually the most organized structures, but I may be wrong.
14 January 2024 4,964 1 View
I need to check DFS in group of patients' with colorectal cancer to research. Can you help me where I can foud that info?
24 August 2023 2,852 1 View
Self-Leadership Questionnaire
05 March 2023 3,116 2 View
Which softwares do you recommend for the analysis of SNPs whether they are binding targets of miRNA or binding sites of proteins?
07 February 2023 6,577 0 View
I am planning to analyze different compounds on SH-SY5Y. What is the best control line for this kind of experiment?
14 September 2022 9,542 1 View
Hi, I’m wondering what statistical test to perform. I aim to measure sex differences in attitude towards two different types of movies. Each participant will complete the attitude test in two...
14 April 2022 1,769 5 View
I am writing a review on vegetables' phytochemical content, andd I am reporting values in mg / 100 g dry weight. I am not an expert on food analysis neither food chemistry. I have found an article...
12 March 2021 274 12 View
I need MRI images database of brain in DICOM to bachelor thesis. Maybe someone can share me some database or reccomend website where I can find dicom files. I tried to find something on...
21 July 2020 5,257 2 View
Al preguntar cuáles son las variables psicológicas deseo saber si se han realizado investigaciones sobre las variables o factores psicológicos que intervienen en la generación y mantenimiento de...
09 July 2019 711 2 View
I tested the activity of some prepared formulations of mosquitocidal bacilli through 10 months storage. these formulations was 40 and i tested the activity of these formulations every month...
17 November 2018 9,034 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View