15 September 2022 1 10K Report

I am planning to analyze different compounds on SH-SY5Y. What is the best control line for this kind of experiment?

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How to check DFS in group of patines witc colorectal cancer?

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Types of vertical wind turbines ?

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Which softwares do you recommend for the analysis of SNPs whether they are binding targets of miRNA or binding sites of proteins?

Which softwares do you recommend for the analysis of SNPs whether they are binding targets of miRNA or binding sites of proteins?

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Which statistical test would be best to perform? MANOVA or ANOVA?

Hi, I’m wondering what statistical test to perform. I aim to measure sex differences in attitude towards two different types of movies. Each participant will complete the attitude test in two...

14 April 2022 1,769 5 View

How to convert from mg GAE/ g extract to mg GAE/ g dry weight?

I am writing a review on vegetables' phytochemical content, andd I am reporting values in mg / 100 g dry weight. I am not an expert on food analysis neither food chemistry. I have found an article...

12 March 2021 274 12 View

Can anyone suggest a MRI images database of a brain in DICOM?

I need MRI images database of brain in DICOM to bachelor thesis. Maybe someone can share me some database or reccomend website where I can find dicom files. I tried to find something on...

21 July 2020 5,257 2 View

¿cuáles son las variables psicológicas del capital social?

Al preguntar cuáles son las variables psicológicas deseo saber si se han realizado investigaciones sobre las variables o factores psicológicos que intervienen en la generación y mantenimiento de...

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Which statistical analysis is suitable to analyse 40 treatments on mortality of larvae through ten months?

I tested the activity of some prepared formulations of mosquitocidal bacilli through 10 months storage. these formulations was 40 and i tested the activity of these formulations every month...

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Similar questions and discussions
Co-culture of SH-SY5Y cells and astrocytes?

Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...

01 July 2024 243 2 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

Which cell line will be the reference for human neuroblastoma?

We are implementing a grant related to the study of supertargets in secondary cultures of human neuroblastoma cells - IMR-32, Kelly, SH-SY5Y, SK-N-AS etc. Comparing gene expression between cell...

16 April 2024 7,706 2 View

SH-SY5Y total cholesterol?

I'm currently doing total cholesterol assay in SH-SY5Y cells treated with 0, 25, 50, 100ug cholesterol/mL (using cholesterol:mbcd, sigma) Lipid extraction is done by chloroform and methanol. After...

12 April 2024 9,353 3 View

Why does the SH-SY5Y cell line can undergo an automatisation of differentiating in a regular growth media after being cryopreserved?

I have cryopreserved the undifferentiated condition of SH-SY5Y cell line in the early passage in a cyomedia formulated with 90% FBS and 10% DMSO (sterile-filtered). Some months later, I have...

25 March 2024 1,152 2 View

Treat MPP+ iodide in SH-SY5Y?

I prepared MPP+ iodide dissolved in water and its stock concentration is 10M. I stored them at -20℃. Then I treated MPP+ 20mM, 10mM, 5mM, 2.5mM for 24H in SH-SY5Y cells. But they don't seem like...

16 March 2024 7,877 1 View

How to achieve RNA extraction of nucleus and cytoplasm in SH-SY5Y cells?

I have tried to achieve a separation of the nucleus and cytoplasm by using RLN buffer+ NP40 (0.2%), and then proceeding with GeneMATRIX universal RNA purification kit (the same used for total RNA...

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Is there any recommended method for growing SH-SY5Y cells on Poly-D-lysine or Poly-L-lysine treated coverslips?

I am experiencing difficulties seeding SH-SY5Y cells on Poly-D-Lysine or Poly-L-Lysine treated coverslips.After 2-3 days, the cells either detach or fail to attain their typical morphology. The...

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Protein extraction from SH-SY5Y neuroblastoma cells and differentiated neurons?

I am working with SH-SY5Y cells and differentiating them into neurons using retinoic acid. I need to extract protein from them in order to conduct a western blot, however, I am having trouble...

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Rotenone doesn't seem cytotoxic on SH-SY5Y and why?

My stock solution of Rotenone (Sigma) is 10mM in which 0.004g of Rot is diluted by 1mL of DMSO. I have done cck-8 assay on SH-SY5Y (2 x 10^4) with Rot treatments. Several articles show that 1uM of...

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