How stable are TCEP-reduced oligos (5'S-S modifier) once purified?

Hi there, I attempted to conjugate some short (~20bp) oligo probes using a commercial kit, but I believe the conjugation failed due to the use of an incorrect filter size. I still have the...

12 August 2019 7,390 1 View

How to measure hydrogen gas in high concentrations without using GC or RGA?

I'm trying to find a lower-cost method of measuring hydrogen gas concentrations in the region of 70-100%. Ideally some sort of sensor that can be used in a oxygen environment (which I am led to...

16 December 2018 471 2 View

Are there any tools available for primer design using a degenerate DNA template?

I'm currently trying to design a set of degenerate primers to amplify an identified conserved region among our target proteins, but I can't find any tool that accepts non-ATCGN degenerate...

26 October 2017 5,828 2 View

Is there a standard etiquette to utilizing surveys built by other researchers, especially if you modify them?

I am building a social support survey utilizing three established surveys, and adding three of my own questions. I am tailoring this survey to examine a specific population of college students...

09 December 2015 2,206 4 View

What are a few good strengths-based assessment tools?

10 November 2015 6,899 6 View

Does the qPCR reaction volume overall effect on CT?

I am struggling right now with my one-step qPCR for my two GOIs. They seem to amplify at a very high CT, and this leads to an impossibility in getting a decent standard curve. At full...

23 June 2014 8,785 8 View

Can you help me with Amplification in NTC (only for some primers) RT-qPCR?

Okay, I know the obvious answer is primer dimers, but I looked at the melt curves, and I am seeing the expected Tm. But I have been getting amplification in my NTC controls in my qPCR. The Cts...

19 June 2014 1,028 2 View

Diluting RNA for one-step qPCR

I was struggling with the standard curves for my RT-qPCR, and my PI suggested trying one-step instead of 2-step. After diluting one of my RNA samples to generate a standard curve (started with...

03 June 2014 1,157 1 View

Can anyone offer advice on using qPCR to compare homolog expression?

Quick background: I want to compare the expression of two homologs in potato. The two genes are >80% identical, so designing primers has to be a tricky process. I have designed a few of my own,...

13 May 2014 4,353 7 View

Should I continue to use Betaine in my master mix?

I have been using Betaine for a while now in my master mix, but I am beginning to question its effectiveness. I know it supposedly serves a purpose to improve target specificity, but there are...

02 April 2014 4,438 3 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View